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Literature summary for 1.13.11.15 extracted from

  • Gibello, A.; Ferrer, E.; Martin, M.; Garrido-Pertierra, A.
    3,4-Dihydroxyphenylacetate 2,3-dioxygenase from Klebsiella pneumoniae, a Mg2+-containing dioxygenase involved in aromatic catabolism (1994), Biochem. J., 301, 145-150.
No PubMed abstract available
Search for more literature for 1.13.11.15

Inhibitors

Inhibitors Comment Organism Structure
3,4-dichlorophenylacetate 5 mM, 50% inhibition Klebsiella pneumoniae
3,4-dihydroxybenzoate 1 mM, 75% inhibition Klebsiella pneumoniae
Cu2+ 0.01 mM, 80% inhibition Klebsiella pneumoniae
H2O2 10 mM; immediate loss of activity Klebsiella pneumoniae
succinate semialdehyde 5 mM 65% inhibition Klebsiella pneumoniae
Zn2+ 0.01 mM, complete inhibition Klebsiella pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.074
-
 3,4-dihydroxybenzoic acid
-
 Klebsiella pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
Iron less than 0.01 gatom Fe2+ per mol of enzyme Klebsiella pneumoniae
Mg2+ 1.1-1.3 gatom of Mg2+ per mol of enzyme, essential for activity Klebsiella pneumoniae
Mn2+ 0.01-0.3 gatom of Mn2+ per mol of enzyme Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25500
-
 4 * 25500, SDS-PAGE Klebsiella pneumoniae
102000
-
 gel filtration Klebsiella pneumoniae

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
102
-
-
 Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4-dihydroxybenzoic acid + O2
-
 Klebsiella pneumoniae 2-hydroxy-5-carboxymuconate semialdehyde
-
 ?
3,4-dihydroxyphenylacetate + O2
-
 Klebsiella pneumoniae 2-hydroxy-5-carboxymethylmuconate semialdehyde
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 25500, SDS-PAGE Klebsiella pneumoniae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Klebsiella pneumoniae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
-
 pH 7.5, 0.1 M sodium potassium phosphate, 1 mM DTT, complete loss of activity after 48 h Klebsiella pneumoniae
40
-
 half-life: 13 min Klebsiella pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Klebsiella pneumoniae

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5
-
 maximal stability at Klebsiella pneumoniae