Protein Variants | Comment | Organism |
---|---|---|
H200A | KM-value for 3,4-dihydroxyphenylacetate is 1.75fold lower than wild-type value, kcat for 3,4-dihydroxyphenylacetate is 69fold lower than wild-type value | Arthrobacter globiformis |
H200E | KM-value for 3,4-dihydroxyphenylacetate is 1.2fold lower than wild-type value, kcat for 3,4-dihydroxyphenylacetate is more than 276fold lower than wild-type value, Mn content per monomer is 2fold lower than in wild-type enzyme | Arthrobacter globiformis |
H200N | KM-value for 3,4-dihydroxyphenylacetate is identical to wild-type value, kcat for 3,4-dihydroxyphenylacetate is 1.73fold lower than wild-type value, Mn content per monomer is 65% of wild-type value | Arthrobacter globiformis |
H200Q | KM-value for 3,4-dihydroxyphenylacetate is 1.2fold lower than wild-type value, kcat for 3,4-dihydroxyphenylacetate is 1.3fold lower than wild-type value | Arthrobacter globiformis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-nitrocatechol | - |
Arthrobacter globiformis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
3,4-Dihydroxyphenylacetate | 23°C, pH 7.5, mutant enzyme H200A | Arthrobacter globiformis | |
0.017 | - |
3,4-Dihydroxyphenylacetate | 23°C, pH 7.5, mutant enzyme H200E | Arthrobacter globiformis | |
0.018 | - |
3,4-Dihydroxyphenylacetate | 23°C, pH 7.5, mutant enzyme H200Q | Arthrobacter globiformis | |
0.021 | - |
3,4-Dihydroxyphenylacetate | 23°C, pH 7.5, mutant enzyme H200N | Arthrobacter globiformis | |
0.021 | - |
3,4-Dihydroxyphenylacetate | 23°C, pH 7.5, wild-type enzyme | Arthrobacter globiformis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn | wild-type enzyme amd mutant enzymes H200A and H200Q: 0.6 Mn per monomer. Mutant enzyme H200E: 0.3 Mn per monomer. Mutent enzyme H200N: 0.4 MN per monomer | Arthrobacter globiformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter globiformis | - |
CM-2 | - |
Arthrobacter globiformis CM-2 | - |
CM-2 | - |
Purification (Comment) | Organism |
---|---|
wild-type enzyme and H200 mutants | Arthrobacter globiformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxyphenylacetate + O2 | - |
Arthrobacter globiformis | 2-hydroxy-5-carboxymethylmuconate semialdehyde | - |
? | |
3,4-dihydroxyphenylacetate + O2 | - |
Arthrobacter globiformis CM-2 | 2-hydroxy-5-carboxymethylmuconate semialdehyde | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Mn(II)-dependent 3,4-dihydroxyphenylacetate 2,3-dioxygenase | - |
Arthrobacter globiformis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.067 | - |
3,4-Dihydroxyphenylacetate | 23°C, pH 7.5, mutant enzyme H200A | Arthrobacter globiformis | |
2.67 | - |
3,4-Dihydroxyphenylacetate | 23°C, pH 7.5, mutant enzyme H200N | Arthrobacter globiformis | |
3.6 | - |
3,4-Dihydroxyphenylacetate | 23°C, pH 7.5, mutant enzyme H200Q | Arthrobacter globiformis | |
4.6 | - |
3,4-Dihydroxyphenylacetate | 23°C, pH 7.5, wild-type enzyme | Arthrobacter globiformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | mutant enzyme H200N and H200Q | Arthrobacter globiformis |
8 | 9 | wild-type enzyme | Arthrobacter globiformis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Ki-values for 4-nitrocatechol for the H200A, H200E, H200N and H200Q variants are between 0.015 and 0.025 mM | Arthrobacter globiformis |