Crystallization (Comment) | Organism |
---|---|
crystallizations of wild-type FeHPCD and mutant Y257F with bound substrates 3,4-dihydroxyphenylacetate and 4-nitrocatechol, hanging drop vapour diffusion method, in either 13% PEG 6000, 0.1 M calcium chloride, 0.1 M Tris-HCl, pH 6.5-7.4 or in 18% PEG 8000, 0.1 M calcium acetate, 0.1 M sodium cacodylate, pH 6.5, at 20°C, cryoprotectant solution containing either 20-25% PEG400 or 20% glycerol in the mother liquor solution, X-ray diffraction structure determination and analysis at 1.50-1.60 A resolution | Brevibacterium fuscum |
Protein Variants | Comment | Organism |
---|---|---|
Y257F | site-directed mutagenesis, changes in kinetics for Y257F variant can be attributed to the loss of interactions between substrate and Tyr257, substrate binding structures compared to the wild-type enzyme, overview | Brevibacterium fuscum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | enzyme-bound, required for catalysis | Brevibacterium fuscum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxyphenylacetate + O2 | Brevibacterium fuscum | - |
2-hydroxy-5-carboxymethylmuconate semialdehyde | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brevibacterium fuscum | Q45135 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxyphenylacetate + O2 | - |
Brevibacterium fuscum | 2-hydroxy-5-carboxymethylmuconate semialdehyde | - |
? | |
4-nitrocatechol + O2 | - |
Brevibacterium fuscum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FeHPCD | - |
Brevibacterium fuscum |
homoprotocatechuate 2,3-dioxygenase | - |
Brevibacterium fuscum |