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Literature summary for 1.13.11.2 extracted from

  • Kachhy, A.N.; Modi, V.V.
    Properties of catachol-2,3-oxygenase from Pseudomonas aeruginosa (1976), Indian J. Biochem. Biophys., 13, 234-236.
    View publication on PubMed
Search for more literature for 1.13.11.2

General Stability

General Stability Organism
molarity of buffer beyond 0.04 M not suitable Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
acetone
-
 Pseudomonas aeruginosa
ATP
-
 Pseudomonas aeruginosa
Mg2+
-
 Pseudomonas aeruginosa
Na2B4O7
-
 Pseudomonas aeruginosa
p-chloromercuribenzoate
-
 Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
catechol + O2 Pseudomonas aeruginosa metacleavage enzyme of catechol metabolism 2-hydroxymuconate semialdehyde
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Pseudomonas aeruginosa

Storage Stability

Storage Stability Organism
0-4°C, 0.02 M Tris-HCl, pH 8.0, fairly stable Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
catechol + O2
-
 Pseudomonas aeruginosa 2-hydroxymuconate semialdehyde
-
 ?
catechol + O2 metacleavage enzyme of catechol metabolism Pseudomonas aeruginosa 2-hydroxymuconate semialdehyde
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 20 min, stable Pseudomonas aeruginosa
50
-
 17 min, stable Pseudomonas aeruginosa
55
-
 5 min, stable Pseudomonas aeruginosa
60
-
 immediate loss of activity Pseudomonas aeruginosa

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5 8 highest stability Pseudomonas aeruginosa