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Literature summary for 1.13.11.2 extracted from
- Catechol 2,3-dioxygenases from Pseudomonas aeruginosa 2x (1990), Methods Enzymol., 188, 115-121.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Pseudomonas aeruginosa |  |
| CuCl | - |
Pseudomonas aeruginosa | |
| CuSO4 | - |
Pseudomonas aeruginosa | |
| o-phenanthroline | - |
Pseudomonas aeruginosa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
catechol | metapyrocatechase 2 | Pseudomonas aeruginosa | |
| 0.0041 | - |
catechol | metapyrocatechase 1 | Pseudomonas aeruginosa | |
| 0.008 | - |
4-methylcatechol | metapyrocatechase 2 | Pseudomonas aeruginosa | |
| 0.01 | - |
3-methylcatechol | metapyrocatechase 2 | Pseudomonas aeruginosa | |
| 0.02 | - |
4-methylcatechol | metapyrocatechase 1 | Pseudomonas aeruginosa | |
| 0.06 | - |
3-methylcatechol | metapyrocatechase 1 | Pseudomonas aeruginosa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | FeCl2 and FeSO4 activate metapyrocatechase 2, no effect on metapyrocatechase 1 | Pseudomonas aeruginosa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33500 | - |
4 * 33500 metapyrocatechase 1, SDS-PAGE | Pseudomonas aeruginosa |
| 36000 | - |
4 * 36000, metapyrocatechase 2, SDS-PAGE | Pseudomonas aeruginosa |
| 132000 | 134000 | gel filtration, sedimentation equilibrium analysis, amino acid analysis, metapyrocatechase 2 | Pseudomonas aeruginosa |
| 143000 | 144600 | gel filtration, sedimentation equilibrium analysis, amino acid analysis | Pseudomonas aeruginosa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| catechol + O2 | Pseudomonas aeruginosa | metapyrocatechase 1 takes part in oxidation of aromatic compounds, producing catechol as an intermediate, metapyrocatechase 2 is involved in oxidation of methyl-substituted aromatic substrates | 2-hydroxymuconate semialdehyde | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| metapyrocatechase 1 and 2 | Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 35 | - |
metapyrocatechase 2 | Pseudomonas aeruginosa |
| 90 | - |
metapyrocatechase 1 | Pseudomonas aeruginosa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, in lyophilized state for several months, metapyrocatechase 1 and 2 | Pseudomonas aeruginosa |
| 4°C, in acetone buffer under nitrogen for several weeks, metapyrocatechase 1 and 2 | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,4-dihydroxybenzoate + O2 | weak | Pseudomonas aeruginosa | ? | - |
? | |
| 3-chlorocatechol + O2 | weak | Pseudomonas aeruginosa | 2-chloro-2-hydroxy-6-oxohexa-2,4-dienoate | - |
? | |
| 3-methylcatechol + O2 | weak | Pseudomonas aeruginosa | 2-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
| 4-methylcatechol + O2 | - |
Pseudomonas aeruginosa | 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate | - |
? | |
| catechol + O2 | - |
Pseudomonas aeruginosa | 2-hydroxymuconate semialdehyde | - |
? | |
| catechol + O2 | metapyrocatechase 1 takes part in oxidation of aromatic compounds, producing catechol as an intermediate, metapyrocatechase 2 is involved in oxidation of methyl-substituted aromatic substrates | Pseudomonas aeruginosa | 2-hydroxymuconate semialdehyde | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 33500 metapyrocatechase 1, SDS-PAGE | Pseudomonas aeruginosa |
| tetramer | 4 * 36000, metapyrocatechase 2, SDS-PAGE | Pseudomonas aeruginosa |
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