Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | - |
Pseudomonas sp. | |
CuSO4 | - |
Pseudomonas sp. | |
Hg2+ | - |
Pseudomonas sp. | |
o-phenanthroline | not | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
3-methylcatechol | - |
Pseudomonas sp. | |
0.0039 | - |
catechol | - |
Pseudomonas sp. | |
0.0109 | - |
4-methylcatechol | - |
Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 0.6 mol of iron per mol of protein | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
4 * 33000 | Pseudomonas sp. |
110000 | - |
gel filtration | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
catechol + O2 | Pseudomonas sp. | catechol 2,3-dioxygenase is a key enzyme in the catabolism of monocyclic aromatic compounds including aniline and catalyzes the extradiol cleavage of catechol | 2-hydroxymuconate semialdehyde | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pseudomonas sp. |
Storage Stability | Organism |
---|---|
4°C, buffer, containing 5% acetone, pH 6-7,5, 24 h, stable | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methylcatechol + O2 | - |
Pseudomonas sp. | 2-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
4-chlorocatechol + O2 | weak | Pseudomonas sp. | 5-chloro-2-hydroxymuconic semialdehyde | - |
? | |
4-methylcatechol + O2 | - |
Pseudomonas sp. | 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate | - |
? | |
catechol + O2 | - |
Pseudomonas sp. | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | catechol 2,3-dioxygenase is a key enzyme in the catabolism of monocyclic aromatic compounds including aniline and catalyzes the extradiol cleavage of catechol | Pseudomonas sp. | 2-hydroxymuconate semialdehyde | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 33000 | Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
10 min, activity not decreased in the presence of 5% acetone | Pseudomonas sp. |
75 | - |
40% loss of original activity | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | - |
Pseudomonas sp. |