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Literature summary for 1.13.11.2 extracted from

  • Murakami, S.; Nakanishi, Y.; Kodama, N.; Takenaka, S.; Shinke, R.; Aoki, K.
    Purification, characterization, and gene analysis of catechol 2,3-dioxygenase from the aniline-assimilating bacterium Pseudomonas species AW-2 (1998), Biosci. Biotechnol. Biochem., 62, 747-752.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
Pseudomonas sp.
CuSO4
-
Pseudomonas sp.
Hg2+
-
Pseudomonas sp.
o-phenanthroline not Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
3-methylcatechol
-
Pseudomonas sp.
0.0039
-
catechol
-
Pseudomonas sp.
0.0109
-
4-methylcatechol
-
Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 0.6 mol of iron per mol of protein Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
4 * 33000 Pseudomonas sp.
110000
-
gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
catechol + O2 Pseudomonas sp. catechol 2,3-dioxygenase is a key enzyme in the catabolism of monocyclic aromatic compounds including aniline and catalyzes the extradiol cleavage of catechol 2-hydroxymuconate semialdehyde
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Pseudomonas sp.

Storage Stability

Storage Stability Organism
4°C, buffer, containing 5% acetone, pH 6-7,5, 24 h, stable Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methylcatechol + O2
-
Pseudomonas sp. 2-hydroxy-6-oxohepta-2,4-dienoate
-
?
4-chlorocatechol + O2 weak Pseudomonas sp. 5-chloro-2-hydroxymuconic semialdehyde
-
?
4-methylcatechol + O2
-
Pseudomonas sp. 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate
-
?
catechol + O2
-
Pseudomonas sp. 2-hydroxymuconate semialdehyde
-
?
catechol + O2 catechol 2,3-dioxygenase is a key enzyme in the catabolism of monocyclic aromatic compounds including aniline and catalyzes the extradiol cleavage of catechol Pseudomonas sp. 2-hydroxymuconate semialdehyde
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 33000 Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
10 min, activity not decreased in the presence of 5% acetone Pseudomonas sp.
75
-
40% loss of original activity Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
Pseudomonas sp.