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Literature summary for 1.13.11.2 extracted from

  • Ishida, T.; Tanaka, H.; Horiike, K.
    Quantitative structure-activity relationship for the cleavage of C3/C4-substituted catechols by a prototypal extradiol catechol dioxygenase with broad substrate specificity (2004), J. Biochem., 135, 721-730.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
catechol + O2 Pseudomonas putida degradation of aromatic compounds 2-hydroxymuconate semialdehyde
-
?
catechol + O2 Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 degradation of aromatic compounds 2-hydroxymuconate semialdehyde
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida P06622
-
-
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 P06622
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-

Storage Stability

Storage Stability Organism
4°C, 10 mM HEPES, pH 7.5, 150 mM NaCl, aerobic conditions, stable for several weeks Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-chlorocatechol + O2 rapid inactivation of enzyme during turnover Pseudomonas putida 3-chloro-2-hydroxymuconate semialdehyde
-
?
3-chlorocatechol + O2 rapid inactivation of enzyme during turnover Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 3-chloro-2-hydroxymuconate semialdehyde
-
?
3-formylcatechol + O2
-
Pseudomonas putida 3-formyl-2-hydroxymuconate semialdehyde
-
?
3-formylcatechol + O2
-
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 3-formyl-2-hydroxymuconate semialdehyde
-
?
3-phenylcatechol + O2
-
Pseudomonas putida 2-hydroxy-3-phenylmuconate semialdehyde
-
?
4-bromocatechol + O2
-
Pseudomonas putida 4-bromo-2-hydroxymuconate semialdehyde
-
?
4-chlorocatechol + O2
-
Pseudomonas putida 4-chloro-2-hydroxymuconate semialdehyde
-
?
4-formylcatechol + O2
-
Pseudomonas putida 4-formyl-2-hydroxymuconate semialdehyde
-
?
4-hydroxymethylcatechol + O2 rapid inactivation of enzyme during turnover Pseudomonas putida 2-hydroxy-4-hydroxymethylmuconate semialdehyde
-
?
4-nitrocatechol + O2
-
Pseudomonas putida 2-hydroxy-4-nitromuconate semialdehyde
-
?
catechol + O2
-
Pseudomonas putida 2-hydroxymuconate semialdehyde
-
?
catechol + O2 degradation of aromatic compounds Pseudomonas putida 2-hydroxymuconate semialdehyde
-
?
catechol + O2
-
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 2-hydroxymuconate semialdehyde
-
?
catechol + O2 degradation of aromatic compounds Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 2-hydroxymuconate semialdehyde
-
?
additional information no cleavage of 4-carboxycatechol, 4-carboxymethylcatechol and 4-tert-butylcatechol Pseudomonas putida ?
-
?
additional information no cleavage of 4-carboxycatechol, 4-carboxymethylcatechol and 4-tert-butylcatechol Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 ?
-
?

Subunits

Subunits Comment Organism
tetramer
-
Pseudomonas putida

Synonyms

Synonyms Comment Organism
metapyrocatechase
-
Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 8.5 depending on the substrate Pseudomonas putida