Cloned (Comment) | Organism |
---|---|
gene c23o, DNA and amino acid sequence determination and analysis of wild-type and mutant enzymes and recombinant expression in Escherichia coli strain BL21 | Planococcus sp. (in: Bacteria) |
Protein Variants | Comment | Organism |
---|---|---|
A23T/F212S | random mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme | Planococcus sp. (in: Bacteria) |
C103R/K289stop | random mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme | Planococcus sp. (in: Bacteria) |
F191I/C268R/Y272H/V280A/Y293D / | random mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme | Planococcus sp. (in: Bacteria) |
G127D/P140T | random mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme | Planococcus sp. (in: Bacteria) |
H24R/F168S/Q275R | random mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme | Planococcus sp. (in: Bacteria) |
L13R | random mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme | Planococcus sp. (in: Bacteria) |
M65T | random mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme | Planococcus sp. (in: Bacteria) |
R296Q | random mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme | Planococcus sp. (in: Bacteria) |
R296Q | random mutagenesis, the mutant shows significant tolerance to acidic pH with an optimum at pH 5.0, as well as a over 1.5fold increased activity, altered substrate inhibition, and 2.5fold lower KM compared to the wild-type enzyme | Planococcus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.037 | - |
catechol | pH 5.0, 35°C, mutant R296Q | Planococcus sp. (in: Bacteria) | |
0.04 | - |
catechol | pH 7.0, 35°C, mutant A23T/F212S | Planococcus sp. (in: Bacteria) | |
0.053 | - |
catechol | pH 7.0, 35°C, mutant H24R/F168S/Q275R | Planococcus sp. (in: Bacteria) | |
0.09 | - |
catechol | pH 7.0, 35°C, wild-type enzyme | Planococcus sp. (in: Bacteria) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
catechol + O2 | Planococcus sp. (in: Bacteria) | - |
2-hydroxymuconate-6-semialdehyde | - |
? | |
catechol + O2 | Planococcus sp. (in: Bacteria) S5 | - |
2-hydroxymuconate-6-semialdehyde | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Planococcus sp. (in: Bacteria) | - |
gene c23o | - |
Planococcus sp. (in: Bacteria) S5 | - |
gene c23o | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00887 | - |
pH 7.0, 35°C, mutant H24R/F168S/Q275R | Planococcus sp. (in: Bacteria) |
0.01285 | - |
pH 7.0, 35°C, mutant A23T/F212S | Planococcus sp. (in: Bacteria) |
0.01973 | - |
pH 7.0, 35°C, wild-type enzyme | Planococcus sp. (in: Bacteria) |
0.03187 | - |
pH 5.0, 35°C, mutant R296Q | Planococcus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
catechol + O2 | - |
Planococcus sp. (in: Bacteria) | 2-hydroxymuconate-6-semialdehyde | - |
? | |
catechol + O2 | - |
Planococcus sp. (in: Bacteria) S5 | 2-hydroxymuconate-6-semialdehyde | - |
? |
Synonyms | Comment | Organism |
---|---|---|
C23o | - |
Planococcus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
wild-type and mutant enzymes | Planococcus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
mutant R296Q | Planococcus sp. (in: Bacteria) |
6.5 | - |
mutants A23T/F212S and H24R/F168S/Q275R | Planococcus sp. (in: Bacteria) |
7 | - |
wild-type enzyme | Planococcus sp. (in: Bacteria) |
General Information | Comment | Organism |
---|---|---|
additional information | the enzyme is a member of the superfamily I, subfamily 2A of extradiol dioxygenases | Planococcus sp. (in: Bacteria) |
physiological function | catechol 2,3-dioxygenases play a role in the degradation of monoaromatic hydrocarbons | Planococcus sp. (in: Bacteria) |