Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NADH | stimulates | Histoplasma capsulatum |
General Stability | Organism |
---|---|
pronase destroys activity | Histoplasma capsulatum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | totally inhibits at very low concentrations | Histoplasma capsulatum | |
EGTA | totally inhibits at very low concentrations | Histoplasma capsulatum | |
o-phenanthroline | totally inhibits at very low concentrations | Histoplasma capsulatum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
L-cysteine | - |
Histoplasma capsulatum | |
16.6 | - |
beta-mercaptoethanol | - |
Histoplasma capsulatum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Histoplasma capsulatum | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | stimulation, restores activity after inhibition with EDTA | Histoplasma capsulatum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
10500 | - |
gel filtration, SDS-PAGE, the estimate of molecular weight may be inaccurate because it is based on use of the iodinated protein and the assumption that iodination does not affect molecular weight | Histoplasma capsulatum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cysteine + O2 | Histoplasma capsulatum | probable role in the mycelial to yeast phase transition | 3-sulfino-L-alanine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Histoplasma capsulatum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using filtration, centrifugation, column chromatography on DEAE-cellulose, Sephadex G-50 and cysteine-Sepharose | Histoplasma capsulatum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | no activity in mycelium | Histoplasma capsulatum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
6.31 atoms of oxygen/1000000 * min * mg protein | Histoplasma capsulatum |
Storage Stability | Organism |
---|---|
-70°C, crude enzyme, stable for up to 4 weeks | Histoplasma capsulatum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-mercaptoethanol + O2 | slight activity | Histoplasma capsulatum | 2-hydroxyethanesulfinate | - |
? | |
L-cysteine + O2 | highly specific for L-cysteine | Histoplasma capsulatum | 3-sulfino-L-alanine | - |
? | |
L-cysteine + O2 | probable role in the mycelial to yeast phase transition | Histoplasma capsulatum | 3-sulfino-L-alanine | - |
? | |
additional information | D-cysteine, cystine, taurine, cystamine, cysteinesulfinic acid, glutathione, cysteic acid, S-methylcysteine and pyruvic acid do not serve as substrates | Histoplasma capsulatum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 10500, SDS-PAGE | Histoplasma capsulatum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
activity at 37°C 2-fold higher than at 25°C | Histoplasma capsulatum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 37 | activity at 37°C 2-fold higher than at 25°C | Histoplasma capsulatum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Histoplasma capsulatum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 1 mol of enzyme contains about 0.1 mol of flavin | Histoplasma capsulatum |