Literature summary for 1.13.11.20 extracted from

Ye, S.; Wu, X.; Wei, L.; Tang, D.; Sun, P.; Bartlam, M.; Rao, Z.
An insight into the mechanism of human cysteine dioxygenase. Key roles of the thioether-bonded tyrosine-cysteine cofactor (2007), J. Biol. Chem., 282, 3391-3402.

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Cloned(Commentary)

Cloned (Comment)	Organism
cloned into pGEX-6p-1 expression vector, expressed in Escherichia coli Bl21(DE3), glutathione S-transferase-fused CDO is purified and GTS tag is removed by cleavage with PreScission Protease	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
co-crystallization of L-cysteine and purified wild-type cysteine dioxygenase by the hanging drop, vapor-diffusion method at 16°C, the crystals grow as rods up to 0.2 x 0.2 x 0.8 mm in 1 week. Refinement of the crystal stucture leads to a final model with a crystallographic R-factor of 18.1% and a free R-factor of 21.5% at 2.7 A resolution.	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
C164A	mutation of C164 shows a about 20% abatement of enzymatic activity	Homo sapiens
C164S	mutation of C164 shows a about 20% abatement of enzymatic activity	Homo sapiens
C93A	C93 mutation reduces activity to 50%, zinc content of about 45%, specific activity of Cys-93 mutants is proportional to the measured iron content.	Homo sapiens
C93S	C93 mutation reduces activity to 50%, zinc content of about 45%, specific activity of Cys-93 mutants is proportional to the measured iron content.	Homo sapiens
R60A	similar results as R60Q, R60 mutation reduces activity to 30%	Homo sapiens
R60Q	R60 mutation reduces activity to 30%	Homo sapiens
Y157F	in the gel-filtration chromatography Y157F shows an additional peak with an estimated molecular weight equivalent to a cysteine dioxygenase dimer. The results for monomer and dimer are similar. Activity reduced to 5% of the wild type activity. Zinc content of about 45%	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	an EDTA:cysteine dioxygenase molar ratio of about 1000:1 abolish`s cysteine oxidase activity	Homo sapiens
homocysteine	cysteine dioxygenase activity is reduced by 50% only when the molar ratio of homocysteine:cysteine dioxygenase reach about 30000:1	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.1	-	L-cysteine	under conditions where the second substrate oxygen is saturated	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	purified human cystein dioxygenase yields a iron incorporation of about 68%	Homo sapiens
Fe2+	unsaturated distored tetragonal bipyramidal ferrous center with a vacant coordination site	Homo sapiens
Mg	purified human cystein dioxygenase yields trace amounts of magnesium about 0.41%	Homo sapiens
Mn	purified human cystein dioxygenase yields trace amounts of manganese about 0.25%	Homo sapiens
Zn2+	purified human cystein dioxygenase yields a zinc incorporation of about 18.1%	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q16878	-	-

Purification (Commentary)

Purification (Comment)	Organism
standard glutathione S-transferase fusion protein purification protocol, gel-filtration chromatography after removing the glutathione S-transferase tag	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-cysteine + O2	-	Homo sapiens	3-sulfino-L-alanine	-	?

Synonyms

Synonyms	Comment	Organism
CDO	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.7	-	L-cysteine	determind under saturating oxygen conditions	Homo sapiens

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Release 2023.1 (January 2023)