Cloned (Comment) | Organism |
---|---|
- |
Picrophilus torridus |
Crystallization (Comment) | Organism |
---|---|
crystals are grown by sitting-drop vapor diffusion method at 20°C | Picrophilus torridus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 10 mM, 65% inhibition | Picrophilus torridus | |
4-hydroxyphenylpyruvate | substrate inhibition | Picrophilus torridus | |
Al3+ | 10 mM, 7% inhibition | Picrophilus torridus | |
Ca2+ | 10 mM, 16% inhibition | Picrophilus torridus | |
Co2+ | 10 mM, 32% inhibition | Picrophilus torridus | |
Cu2+ | 10 mM, complete inhibition | Picrophilus torridus | |
DTT | 10 mM, 61% inhibition | Picrophilus torridus | |
EDTA | 10 mM, 76% inhibition | Picrophilus torridus | |
Fe2+ | 10 mM, 82% inhibition | Picrophilus torridus | |
K+ | 10 mM, 6% inhibition | Picrophilus torridus | |
Mg2+ | 10 mM, 18% inhibition | Picrophilus torridus | |
Mn2+ | 10 mM, 97% inhibition | Picrophilus torridus | |
Na+ | 10 mM, 14% inhibition | Picrophilus torridus | |
Ni2+ | 10 mM, 28% inhibition | Picrophilus torridus | |
SDS | 2% (w/v), complete inhibition | Picrophilus torridus | |
Urea | 1 M, 3% inhibition. 3 M, 42% inhibition. 5 M, 56% inhibition | Picrophilus torridus | |
Zn2+ | 10 mM, 65% inhibition | Picrophilus torridus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.142 | - |
4-hydroxyphenylpyruvate | pH 7.0, 50°C | Picrophilus torridus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxyphenylpyruvate + O2 | Picrophilus torridus | second step of oxidative tyrosine catabolism | homogentisate + CO2 | - |
? | |
4-hydroxyphenylpyruvate + O2 | Picrophilus torridus DSM 9790 | second step of oxidative tyrosine catabolism | homogentisate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Picrophilus torridus | Q6KZ98 | - |
- |
Picrophilus torridus DSM 9790 | Q6KZ98 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Picrophilus torridus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxyphenylpyruvate + O2 | - |
Picrophilus torridus | homogentisate + CO2 | - |
? | |
4-hydroxyphenylpyruvate + O2 | second step of oxidative tyrosine catabolism | Picrophilus torridus | homogentisate + CO2 | - |
? | |
4-hydroxyphenylpyruvate + O2 | - |
Picrophilus torridus DSM 9790 | homogentisate + CO2 | - |
? | |
4-hydroxyphenylpyruvate + O2 | second step of oxidative tyrosine catabolism | Picrophilus torridus DSM 9790 | homogentisate + CO2 | - |
? | |
additional information | no activity is observed for 3-hydroxyphenylpyruvate, 3,4-dihydroxyphenylpyruvate, 4-hydroxyphenylglyoxylate, phenylpyruvate, 2-oxoglutarate, glyoxylate, pyruvate, oxaloacetate or oxobutyrate | Picrophilus torridus | ? | - |
? | |
additional information | no activity is observed for 3-hydroxyphenylpyruvate, 3,4-dihydroxyphenylpyruvate, 4-hydroxyphenylglyoxylate, phenylpyruvate, 2-oxoglutarate, glyoxylate, pyruvate, oxaloacetate or oxobutyrate | Picrophilus torridus DSM 9790 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PTO1369 | locus name | Picrophilus torridus |
Pt_Hpd | - |
Picrophilus torridus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
maximal activity when assayed at pH 4.0 or 7.0 | Picrophilus torridus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | 30°C: about 65% of maximal activity, 70°C: about 30% of maximal activity, assayed at pH 7.0 | Picrophilus torridus |
40 | 60 | 40°C: about 55% of maximal activity, 60°C: about 40% of maximal activity, assayed at pH 4.0 | Picrophilus torridus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
pH 4.0, half-life: 1.5 h | Picrophilus torridus |
77 | - |
strong conformational changes are observed at 77°C, indicating melting of the protein | Picrophilus torridus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.9 | - |
4-hydroxyphenylpyruvate | pH 7.0, 50°C | Picrophilus torridus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
two pH optima are observed at 50°C: pH 4.0 (close to native conditions, narrow maximum) and pH 7.0 (broad maximum) | Picrophilus torridus |
7 | - |
two pH optima are observed at 50°C: pH 4.0 (close to native conditions, narrow maximum) and pH 7.0 (broad maximum) | Picrophilus torridus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
4-hydroxyphenylpyruvate | pH 7.0, 50°C | Picrophilus torridus |
General Information | Comment | Organism |
---|---|---|
metabolism | second step of oxidative tyrosine catabolism | Picrophilus torridus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
69.7 | - |
4-hydroxyphenylpyruvate | pH 7.0, 50°C | Picrophilus torridus |