Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-sulfocatechol | competitive vs. protocatechuate | Agrobacterium tumefaciens | |
4-sulfocatechol | competitive vs. protocatechuate | Hydrogenophaga palleronii | |
protocatechuate | competitive vs. 4-sulfocatechol | Agrobacterium tumefaciens | |
protocatechuate | competitive vs. 4-sulfocatechol | Hydrogenophaga palleronii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.042 | - |
4-sulfocatechol | protocatechuate 3,4-dioxygenase type II | Hydrogenophaga palleronii | |
0.054 | - |
protocatechuate | protocatechuate 3,4-dioxygenase type I | Agrobacterium tumefaciens | |
0.058 | - |
protocatechuate | protocatechuate 3,4-dioxygenase type II | Hydrogenophaga palleronii | |
0.058 | - |
4-sulfocatechol | protocatechuate 3,4-dioxygenase type II | Agrobacterium tumefaciens | |
0.205 | - |
protocatechuate | protocatechuate 3,4-dioxygenase type II | Agrobacterium tumefaciens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agrobacterium tumefaciens | - |
protocatechuate 3,4-dioxygenase I and II | - |
Agrobacterium tumefaciens S2 | - |
protocatechuate 3,4-dioxygenase I and II | - |
Hydrogenophaga palleronii | - |
protocatechuate 3,4-dioxygenase II | - |
Hydrogenophaga palleronii S1 | - |
protocatechuate 3,4-dioxygenase II | - |
Purification (Comment) | Organism |
---|---|
Q-Sepharose FF, ammonium sulfate, Sephacryl S-300HR, phenyl-superose HR5/5, Mono Q, type II protocatechuate 3,4-dioxygenase | Agrobacterium tumefaciens |
Q-Sepharose FF, ammonium sulfate, Sephacryl S-300HR, Phenyl-Superose, type I protocatechuate 3,4-dioxygenase | Agrobacterium tumefaciens |
Q-Sepharose FF, ammonium sulfate, Superdex 200, Fractogel, type II protocatechuate 3,4-dioxygenase | Hydrogenophaga palleronii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.2 | - |
type II protocatechuate 3,4-dioxygenase, oxidation of 4-sulfocatechol | Hydrogenophaga palleronii |
16.7 | - |
type II protocatechuate 3,4-dioxygenase, oxidation of 4-sulfocatechol | Agrobacterium tumefaciens |
105 | - |
type I protocatechuate 3,4-dioxygenase | Agrobacterium tumefaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxybenzoate + O2 | - |
Agrobacterium tumefaciens | 3-carboxy-cis,cis-muconate | - |
? | |
3,4-dihydroxybenzoate + O2 | - |
Hydrogenophaga palleronii | 3-carboxy-cis,cis-muconate | - |
? | |
3,4-dihydroxybenzoate + O2 | - |
Agrobacterium tumefaciens S2 | 3-carboxy-cis,cis-muconate | - |
? | |
3,4-dihydroxybenzoate + O2 | - |
Hydrogenophaga palleronii S1 | 3-carboxy-cis,cis-muconate | - |
? | |
3,4-dihydroxymandelic acid + O2 | protocatechuate 3,4-dioxygenase II | Hydrogenophaga palleronii | ? | - |
? | |
3,4-dihydroxymandelic acid + O2 | protocatechuate 3,4-dioxygenase II | Hydrogenophaga palleronii S1 | ? | - |
? | |
3,4-dihydroxyphenylacetate + O2 | - |
Agrobacterium tumefaciens | ? | - |
? | |
3,4-dihydroxyphenylacetate + O2 | - |
Hydrogenophaga palleronii | ? | - |
? | |
3-(3,4-dihydroxyphenyl)propionate + O2 | protocatechuate 3,4-dioxygenase II | Hydrogenophaga palleronii | ? | - |
? | |
3-methylcatechol + O2 | - |
Agrobacterium tumefaciens | 2-methylmuconate | - |
? | |
3-methylcatechol + O2 | - |
Hydrogenophaga palleronii | 2-methylmuconate | - |
? | |
3-methylcatechol + O2 | - |
Agrobacterium tumefaciens S2 | 2-methylmuconate | - |
? | |
3-methylcatechol + O2 | - |
Hydrogenophaga palleronii S1 | 2-methylmuconate | - |
? | |
4-methylcatechol + O2 | at very low rates | Agrobacterium tumefaciens | 3-methyl-cis,cis-muconate | - |
? | |
4-methylcatechol + O2 | at very low rates | Hydrogenophaga palleronii | 3-methyl-cis,cis-muconate | - |
? | |
4-methylcatechol + O2 | at very low rates | Agrobacterium tumefaciens S2 | 3-methyl-cis,cis-muconate | - |
? | |
4-methylcatechol + O2 | at very low rates | Hydrogenophaga palleronii S1 | 3-methyl-cis,cis-muconate | - |
? | |
4-sulfocatechol + O2 | catalyzed by protocatechuate 3,4-dioxygenase type II only | Agrobacterium tumefaciens | 3-sulfomuconate | - |
? | |
4-sulfocatechol + O2 | catalyzed by protocatechuate 3,4-dioxygenase type II only | Hydrogenophaga palleronii | 3-sulfomuconate | - |
? | |
4-sulfocatechol + O2 | catalyzed by protocatechuate 3,4-dioxygenase type II only | Agrobacterium tumefaciens S2 | 3-sulfomuconate | - |
? | |
catechol + O2 | - |
Agrobacterium tumefaciens | muconate | - |
? | |
catechol + O2 | - |
Hydrogenophaga palleronii | muconate | - |
? | |
catechol + O2 | - |
Agrobacterium tumefaciens S2 | muconate | - |
? | |
catechol + O2 | - |
Hydrogenophaga palleronii S1 | muconate | - |
? | |
trans-3,4-dihydroxycinnamate + O2 | - |
Agrobacterium tumefaciens | ? | - |
? | |
trans-3,4-dihydroxycinnamate + O2 | - |
Hydrogenophaga palleronii | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14.6 | - |
4-sulfocatechol | protocatechuate 3,4-dioxygenase type II | Agrobacterium tumefaciens | |
18.8 | - |
protocatechuate | protocatechuate 3,4-dioxygenase type II | Agrobacterium tumefaciens | |
89.5 | - |
protocatechuate | protocatechuate 3,4-dioxygenase type I | Agrobacterium tumefaciens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
4-sulfocatechol | protocatechuate 3,4-dioxygenase type II | Hydrogenophaga palleronii | |
0.053 | - |
4-sulfocatechol | protocatechuate 3,4-dioxygenase type II | Agrobacterium tumefaciens | |
0.066 | - |
protocatechuate | protocatechuate 3,4-dioxygenase type II | Hydrogenophaga palleronii | |
0.102 | - |
protocatechuate | protocatechuate 3,4-dioxygenase type II | Agrobacterium tumefaciens |