Cloned (Comment) | Organism |
---|---|
expression of wild-type and Y447H mutant enzyme in Pseudomonas fluorescens | Pseudomonas putida |
Crystallization (Comment) | Organism |
---|---|
vapor diffusion with ammonium sulfate as the precipitant at 4°C, recombinant wild-type and Y447H mutant enzyme | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
Y447H | greatly reduced rate of protocatechuate oxygenation | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0029 | - |
protocatechuate | Y447H mutant enzyme | Pseudomonas putida | |
0.006 | - |
O2 | Y447H mutant enzyme | Pseudomonas putida | |
0.029 | - |
protocatechuate | recombinant enzyme | Pseudomonas putida | |
0.03 | - |
protocatechuate | native enzyme | Pseudomonas putida | |
0.04 | - |
O2 | native enzyme | Pseudomonas putida | |
0.056 | - |
O2 | recombinant enzyme | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
no activity in Pseudomonas fluorescens | - |
- |
- |
Pseudomonas putida | - |
previously classified as Pseudomonas aeruginosa strain ATCC 23975 | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, DEAE-Sepharose, octyl-Sepharose, Phenyl-sepharose, recombinant enzyme | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
37.2 | - |
recombinant enzyme | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxybenzoate + O2 | - |
Pseudomonas putida | 3-carboxy-cis,cis-muconate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
protocatechuate | Y447H mutant enzyme | Pseudomonas putida | |
68 | - |
protocatechuate | recombinant enzyme | Pseudomonas putida | |
70 | - |
protocatechuate | native enzyme | Pseudomonas putida |