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Literature summary for 1.13.11.3 extracted from

  • Contzen, M.; Burger, S.; Stolz, A.
    Cloning of the genes for a 4-sulphocatechol-oxidizing protocatechuate 3,4-dioxygenase from Hydrogenophaga intermedia S1 and identification of the amino acid residues responsible for the ability to convert 4-sulphocatechol (2001), Mol. Microbiol., 41, 199-205.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of protocatechuate 3,4-dioxygenase II in Escherichia coli Hydrogenophaga palleronii
expression of wild-type, W153V, R142K and R142/W153V double mutant of protocatechuate 3,4-dioxygenase I in Escherichia coli Agrobacterium tumefaciens

Protein Variants

Protein Variants Comment Organism
R142K like wild-type no acticity of mutated protocatechuate 3,4-dioxygenase I with 4-sulfocatechol Agrobacterium tumefaciens
R142K/W153V protocatechuate 3,4-dioxygenase I gain of function mutation, mutant enzyme oxidizes 4-sulfocatechol Agrobacterium tumefaciens
R153V protocatechuate 3,4-dioxygenase I gain of function mutation, mutant enzyme oxidizes 4-sulfocatechol Agrobacterium tumefaciens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21502
-
x * 27097 + x * 21502, deduced from nucleotide sequence Hydrogenophaga palleronii
27097
-
x * 27097 + x * 21502, deduced from nucleotide sequence Hydrogenophaga palleronii

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens
-
-
-
Hydrogenophaga palleronii
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4-dihydroxybenzoate + O2
-
Agrobacterium tumefaciens 3-carboxy-cis,cis-muconate
-
?
3,4-dihydroxybenzoate + O2
-
Hydrogenophaga palleronii 3-carboxy-cis,cis-muconate
-
?
3,4-dihydroxyphenylacetate + O2
-
Hydrogenophaga palleronii ?
-
?
3,4-dihydroxyphenylacetate + O2 W153V protocatechuate 3,4-dioxygenase I mutant enzyme: 13% activity compared to protocatechuate, wild-type protocatechuate 3,4-dioxygenase I: 2% activity compared to protocatechuate Agrobacterium tumefaciens ?
-
?
trans-3,4-dihydroxycinnamate + O2 W153V protocatechuate 3,4-dioxygenase I mutant enzyme: 35% activity compared to protocatechuate, wild-type protocatechuate 3,4-dioxygenase I: 2% activity compared to protocatechuate Agrobacterium tumefaciens ?
-
?

Subunits

Subunits Comment Organism
? x * 27097 + x * 21502, deduced from nucleotide sequence Hydrogenophaga palleronii