Cloned (Comment) | Organism |
---|---|
expression of protocatechuate 3,4-dioxygenase II in Escherichia coli | Hydrogenophaga palleronii |
expression of wild-type, W153V, R142K and R142/W153V double mutant of protocatechuate 3,4-dioxygenase I in Escherichia coli | Agrobacterium tumefaciens |
Protein Variants | Comment | Organism |
---|---|---|
R142K | like wild-type no acticity of mutated protocatechuate 3,4-dioxygenase I with 4-sulfocatechol | Agrobacterium tumefaciens |
R142K/W153V | protocatechuate 3,4-dioxygenase I gain of function mutation, mutant enzyme oxidizes 4-sulfocatechol | Agrobacterium tumefaciens |
R153V | protocatechuate 3,4-dioxygenase I gain of function mutation, mutant enzyme oxidizes 4-sulfocatechol | Agrobacterium tumefaciens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21502 | - |
x * 27097 + x * 21502, deduced from nucleotide sequence | Hydrogenophaga palleronii |
27097 | - |
x * 27097 + x * 21502, deduced from nucleotide sequence | Hydrogenophaga palleronii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agrobacterium tumefaciens | - |
- |
- |
Hydrogenophaga palleronii | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxybenzoate + O2 | - |
Agrobacterium tumefaciens | 3-carboxy-cis,cis-muconate | - |
? | |
3,4-dihydroxybenzoate + O2 | - |
Hydrogenophaga palleronii | 3-carboxy-cis,cis-muconate | - |
? | |
3,4-dihydroxyphenylacetate + O2 | - |
Hydrogenophaga palleronii | ? | - |
? | |
3,4-dihydroxyphenylacetate + O2 | W153V protocatechuate 3,4-dioxygenase I mutant enzyme: 13% activity compared to protocatechuate, wild-type protocatechuate 3,4-dioxygenase I: 2% activity compared to protocatechuate | Agrobacterium tumefaciens | ? | - |
? | |
trans-3,4-dihydroxycinnamate + O2 | W153V protocatechuate 3,4-dioxygenase I mutant enzyme: 35% activity compared to protocatechuate, wild-type protocatechuate 3,4-dioxygenase I: 2% activity compared to protocatechuate | Agrobacterium tumefaciens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 27097 + x * 21502, deduced from nucleotide sequence | Hydrogenophaga palleronii |