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Literature summary for 1.13.11.31 extracted from

  • Richards, K.M.; Marnett, L.J.
    Leukocyte 12-lipoxygenase: Expression, purification, and investigation of the role of methionine residues in turnover-dependent inactivation and 5,8,11,14-eicosatetraynoic acid inhibition (1997), Biochemistry, 36, 6692-6699.
    View publication on PubMed
Search for more literature for 1.13.11.31

Cloned(Commentary)

Cloned (Comment) Organism
enzyme is cloned into the expression vectors pSE280, pSE380, and pSE420. PSE380 yields the highest level of 12-lipoxygenase activity, expression in Escherichia coli Top10 cells Sus scrofa

Protein Variants

Protein Variants Comment Organism
M338L Km-value for arachidonic acid is about 1/2 that of the wild type enzyme Sus scrofa
M367V Km-value for arachidonic acid and turnover number are approximately double that of the wild-type enzyme Sus scrofa
M562L Km-value for arachidonic acid is 77% of that of the wild type enzyme, turnover number is 85% of that of the wild type Sus scrofa

General Stability

General Stability Organism
15-25% loss of activity upon freezing and thawing Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
5,8,11,14-Eicosatetraynoic acid
-
 Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 arachidonic acid mutant enzyme M338L Sus scrofa
0.007
-
 arachidonic acid mutant enzyme M562L Sus scrofa
0.009
-
 arachidonic acid wild type enzyme Sus scrofa
0.018
-
 arachidonic acid mutant enzyme M367V Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
leukocyte
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12
-
-
 Sus scrofa

Storage Stability

Storage Stability Organism
-80°C, stable for at least 4 months Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arachidonate + O2 i.e. 5Z,8Z,11Z,14Z-eicosatetraenoic acid Sus scrofa (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.2
-
 arachidonic acid mutant enzyme M561L Sus scrofa
8.4
-
 arachidonic acid wild type enzyme and mutant enzyme M338L Sus scrofa
18
-
 arachidonic acid mutant enzyme M367V Sus scrofa