Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Gersemia fruticosa |
General Stability | Organism |
---|---|
the enzyme shows higher activity in 50 mM CAPS buffer (pH 10-11) and glycine buffer (pH 8.5-10.5) than in 50 mM Tris-HCl (pH 7-9) | Gersemia fruticosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Gersemia fruticosa | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10 mM, required for activity | Gersemia fruticosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
77000 | - |
SDS-PAGE | Gersemia fruticosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gersemia fruticosa | - |
soft coral | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
11,14,17-eicosatrienoic acid + O2 | poor substrate | Gersemia fruticosa | ? | - |
? | |
adrenic acid + O2 | - |
Gersemia fruticosa | ? | - |
? | |
arachidonate + O2 | - |
Gersemia fruticosa | (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate | - |
? | |
dihomo-gamma linolenic acid + O2 | - |
Gersemia fruticosa | ? | - |
? | |
docosahexaenoic acid + O2 | - |
Gersemia fruticosa | 14-hydroxydocosahexaenoic acid | - |
? | |
eicosapentaenoic acid + O2 | - |
Gersemia fruticosa | 12-hydroxyeicosapentaenoic acid | - |
? | |
gamma linolenic acid + O2 | poor substrate | Gersemia fruticosa | 10-hydroxy-octadeca-(6Z,8E,12Z)-trienoic acid | - |
? | |
linolenic acid + O2 | poor substrate | Gersemia fruticosa | ? | - |
? | |
additional information | no reaction with mead acid and methyl esters of linolenic acid and arachidonate | Gersemia fruticosa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
11R-lipoxygenase | - |
Gersemia fruticosa |
11R-LOX | - |
Gersemia fruticosa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 15 | - |
Gersemia fruticosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
significant activity decrease above 25°C | Gersemia fruticosa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 11 | - |
Gersemia fruticosa |