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Literature summary for 1.13.11.31 extracted from

  • Assimes, T.L.; Knowles, J.W.; Priest, J.R.; Basu, A.; Borchert, A.; Volcik, K.A.; Grove, M.L.; Tabor, H.K.; Southwick, A.; Tabibiazar, R.; Sidney, S.; Boerwinkle, E.; Go, A.S.; Iribarren, C.; Hlatky, M.A.; Fortmann, S.P.; Myers, R.M.; Kuhn, H.; Risch, N.; Quertermous, T.
    A near null variant of 12/15-LOX encoded by a novel SNP in ALOX15 and the risk of coronary artery disease (2008), Atherosclerosis, 198, 136-144.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
gene ALOX15, DNA and amino acid sequence determination and analysis of wild-type and mutant T560M, promoter sequence analysis, genotyping, overview, expression of wild-type and mutant enzymes in Escherichia coli and HEK-297 cells, the mutant shows a low expression level in both systems compared to the wild-type enzyme Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information 12/15-LO knockout mice show reduced monocyte chemoattractant protein MCP-1 expression Mus musculus
T560M naturally occurring enzyme mutant, the mutant shows 20fold reduced catalytic activity, genotyping using 12974 samples, the haplotypes show increased risk of coronary artery disease compared to non-carriers, overview Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 arachidonate + 2 O2 + 2 H+ Mus musculus
-
 (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate + (5Z,8Z,10E,14Z)-(12S)-12-hydroxyeicosa-5,8,10,14-tetraenoate + H2O (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate increases expression of monocyte chemoattractant protein MCP-1 in macrophages, also but less by 15(S)-hydroxyeicosatetranoic acid, and 12(S)-HETE activates NADPH oxidase, overview ?
2 arachidonate + 2 O2 + 2 H+ Mus musculus C57BL/6
-
 (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate + (5Z,8Z,10E,14Z)-(12S)-12-hydroxyeicosa-5,8,10,14-tetraenoate + H2O (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate increases expression of monocyte chemoattractant protein MCP-1 in macrophages, also but less by 15(S)-hydroxyeicosatetranoic acid, and 12(S)-HETE activates NADPH oxidase, overview ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 gene ALOX15
-
Mus musculus
-
 C57BL/6 mice
-
Mus musculus C57BL/6
-
 C57BL/6 mice
-

Source Tissue

Source Tissue Comment Organism Textmining
J-774A.1 cell macrophage cell line Mus musculus
-
macrophage peritoneal Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 arachidonate + 2 O2 + 2 H+
-
 Mus musculus (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate + (5Z,8Z,10E,14Z)-(12S)-12-hydroxyeicosa-5,8,10,14-tetraenoate + H2O (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate increases expression of monocyte chemoattractant protein MCP-1 in macrophages, also but less by 15(S)-hydroxyeicosatetranoic acid, and 12(S)-HETE activates NADPH oxidase, overview ?
2 arachidonate + 2 O2 + 2 H+
-
 Mus musculus (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate + (5Z,8Z,10E,14Z)-(12S)-12-hydroxyeicosa-5,8,10,14-tetraenoate + H2O i.e. 12(S)-HPETE and 12(S)-HETE, the first is the predominant product ?
2 arachidonate + 2 O2 + 2 H+
-
 Mus musculus C57BL/6 (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate + (5Z,8Z,10E,14Z)-(12S)-12-hydroxyeicosa-5,8,10,14-tetraenoate + H2O (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate increases expression of monocyte chemoattractant protein MCP-1 in macrophages, also but less by 15(S)-hydroxyeicosatetranoic acid, and 12(S)-HETE activates NADPH oxidase, overview ?
2 arachidonate + 2 O2 + 2 H+
-
 Mus musculus C57BL/6 (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate + (5Z,8Z,10E,14Z)-(12S)-12-hydroxyeicosa-5,8,10,14-tetraenoate + H2O i.e. 12(S)-HPETE and 12(S)-HETE, the first is the predominant product ?
additional information a bifunctional enzyme exhibiting 12-LO and 15-LO activity Homo sapiens ?
-
 ?
additional information a bifunctional enzyme exhibiting 12-LO and 15-LO activity, the enzyme is also able to catalyze stereoselective oxidation of linoleic acid at position 13 over 9 to preferentially form 13(S)-hydroperoxyoctadienoic acid, which enhances MCP-1 expression, overview Mus musculus ?
-
 ?
additional information a bifunctional enzyme exhibiting 12-LO and 15-LO activity, the enzyme is also able to catalyze stereoselective oxidation of linoleic acid at position 13 over 9 to preferentially form 13(S)-hydroperoxyoctadienoic acid, which enhances MCP-1 expression, overview Mus musculus C57BL/6 ?
-
 ?

Synonyms

Synonyms Comment Organism
12-lipoxygenase
-
 Mus musculus
12/15-lipoxygenase
-
 Mus musculus
12/15-lipoxygenase
-
 Homo sapiens
12/15-LO
-
 Mus musculus
12/15-LO
-
 Homo sapiens