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Literature summary for 1.13.11.31 extracted from

  • Moran, J.L.; Qiu, H.; Turbe-Doan, A.; Yun, Y.; Boeglin, W.E.; Brash, A.R.; Beier, D.R.
    A mouse mutation in the 12R-lipoxygenase, Alox12b, disrupts formation of the epidermal permeability barrier (2007), J. Invest. Dermatol., 127, 1893-1897.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
genotyping, overexpression of His-tagged mutant W633X and wild-type Alox12b proteins in COS7 cells Mus musculus

Protein Variants

Protein Variants Comment Organism
W633X naturally occurring mummy mutation, that introduces a nonsense codon into the C-terminal LOX catalytic domain, which results in truncation of the protein by 68 amino acids, mummy is identified in an ethylnitrosurea mutagenesis screen for autosomal recessive developmental phenotypes, the mutant is catalytically inactive, Alox12b deficiency in mice leads to a lack of epidermal permeability barrier function and perinatal lethality Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
arachidonate methyl ester + O2 Mus musculus mouse Alox12b protein, 12R-LOX, oxygenates arachidonate methyl ester rather than arachidonic acid to 12(R)-hydroperoxyeicosatetraenoic acid, HETE, and to 12(R)-HETE methyl ester (5Z,8Z,10E,14Z)-(12R)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate methyl ester
-
?
arachidonate methyl ester + O2 Mus musculus A/J mouse Alox12b protein, 12R-LOX, oxygenates arachidonate methyl ester rather than arachidonic acid to 12(R)-hydroperoxyeicosatetraenoic acid, HETE, and to 12(R)-HETE methyl ester (5Z,8Z,10E,14Z)-(12R)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate methyl ester
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?
additional information Mus musculus Alox12b deficiency in mice leads to a lack of epidermal permeability barrier function and perinatal lethality ?
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?
additional information Mus musculus A/J Alox12b deficiency in mice leads to a lack of epidermal permeability barrier function and perinatal lethality ?
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?

Organism

Organism UniProt Comment Textmining
Mus musculus O70582
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Mus musculus A/J O70582
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arachidonate methyl ester + O2 mouse Alox12b protein, 12R-LOX, oxygenates arachidonate methyl ester rather than arachidonic acid to 12(R)-hydroperoxyeicosatetraenoic acid, HETE, and to 12(R)-HETE methyl ester Mus musculus (5Z,8Z,10E,14Z)-(12R)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate methyl ester
-
?
arachidonate methyl ester + O2 mouse Alox12b protein, 12R-LOX, oxygenates arachidonate methyl ester rather than arachidonic acid to 12(R)-hydroperoxyeicosatetraenoic acid, HETE Mus musculus (5Z,8Z,10E,14Z)-(12R)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate methyl ester
-
?
arachidonate methyl ester + O2 mouse Alox12b protein, 12R-LOX, oxygenates arachidonate methyl ester rather than arachidonic acid to 12(R)-hydroperoxyeicosatetraenoic acid, HETE, and to 12(R)-HETE methyl ester Mus musculus A/J (5Z,8Z,10E,14Z)-(12R)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate methyl ester
-
?
arachidonate methyl ester + O2 mouse Alox12b protein, 12R-LOX, oxygenates arachidonate methyl ester rather than arachidonic acid to 12(R)-hydroperoxyeicosatetraenoic acid, HETE Mus musculus A/J (5Z,8Z,10E,14Z)-(12R)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate methyl ester
-
?
additional information Alox12b deficiency in mice leads to a lack of epidermal permeability barrier function and perinatal lethality Mus musculus ?
-
?
additional information Alox12b deficiency in mice leads to a lack of epidermal permeability barrier function and perinatal lethality Mus musculus A/J ?
-
?

Synonyms

Synonyms Comment Organism
12R-lipoxygenase
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Mus musculus
12R-LOX
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Mus musculus
Alox12b
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Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Mus musculus