Cloned (Comment) | Organism |
---|---|
genotyping, overexpression of His-tagged mutant W633X and wild-type Alox12b proteins in COS7 cells | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
W633X | naturally occurring mummy mutation, that introduces a nonsense codon into the C-terminal LOX catalytic domain, which results in truncation of the protein by 68 amino acids, mummy is identified in an ethylnitrosurea mutagenesis screen for autosomal recessive developmental phenotypes, the mutant is catalytically inactive, Alox12b deficiency in mice leads to a lack of epidermal permeability barrier function and perinatal lethality | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonate methyl ester + O2 | Mus musculus | mouse Alox12b protein, 12R-LOX, oxygenates arachidonate methyl ester rather than arachidonic acid to 12(R)-hydroperoxyeicosatetraenoic acid, HETE, and to 12(R)-HETE methyl ester | (5Z,8Z,10E,14Z)-(12R)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate methyl ester | - |
? | |
arachidonate methyl ester + O2 | Mus musculus A/J | mouse Alox12b protein, 12R-LOX, oxygenates arachidonate methyl ester rather than arachidonic acid to 12(R)-hydroperoxyeicosatetraenoic acid, HETE, and to 12(R)-HETE methyl ester | (5Z,8Z,10E,14Z)-(12R)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate methyl ester | - |
? | |
additional information | Mus musculus | Alox12b deficiency in mice leads to a lack of epidermal permeability barrier function and perinatal lethality | ? | - |
? | |
additional information | Mus musculus A/J | Alox12b deficiency in mice leads to a lack of epidermal permeability barrier function and perinatal lethality | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | O70582 | - |
- |
Mus musculus A/J | O70582 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonate methyl ester + O2 | mouse Alox12b protein, 12R-LOX, oxygenates arachidonate methyl ester rather than arachidonic acid to 12(R)-hydroperoxyeicosatetraenoic acid, HETE, and to 12(R)-HETE methyl ester | Mus musculus | (5Z,8Z,10E,14Z)-(12R)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate methyl ester | - |
? | |
arachidonate methyl ester + O2 | mouse Alox12b protein, 12R-LOX, oxygenates arachidonate methyl ester rather than arachidonic acid to 12(R)-hydroperoxyeicosatetraenoic acid, HETE | Mus musculus | (5Z,8Z,10E,14Z)-(12R)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate methyl ester | - |
? | |
arachidonate methyl ester + O2 | mouse Alox12b protein, 12R-LOX, oxygenates arachidonate methyl ester rather than arachidonic acid to 12(R)-hydroperoxyeicosatetraenoic acid, HETE, and to 12(R)-HETE methyl ester | Mus musculus A/J | (5Z,8Z,10E,14Z)-(12R)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate methyl ester | - |
? | |
arachidonate methyl ester + O2 | mouse Alox12b protein, 12R-LOX, oxygenates arachidonate methyl ester rather than arachidonic acid to 12(R)-hydroperoxyeicosatetraenoic acid, HETE | Mus musculus A/J | (5Z,8Z,10E,14Z)-(12R)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate methyl ester | - |
? | |
additional information | Alox12b deficiency in mice leads to a lack of epidermal permeability barrier function and perinatal lethality | Mus musculus | ? | - |
? | |
additional information | Alox12b deficiency in mice leads to a lack of epidermal permeability barrier function and perinatal lethality | Mus musculus A/J | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
12R-lipoxygenase | - |
Mus musculus |
12R-LOX | - |
Mus musculus |
Alox12b | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mus musculus |