Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Oryctolagus cuniculus |
Protein Variants | Comment | Organism |
---|---|---|
Y98A | kcat and Km (linoleic acid) decreased compared to wild-type, mutant shows strongly reduced catalytic activity compared to wild-type | Oryctolagus cuniculus |
Y98F | kcat and Km (linoleic acid) increased compared to wild-type | Oryctolagus cuniculus |
Y98R | mutant shows strongly reduced catalytic activity compared to wild-type, mutant does not follow Michaelis-Menten-kinetics. Mutant is strongly inhibited by linoleic acid at concentrations above 0.01 mM | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0125 | - |
linoleic acid | pH 7.4, 20°, mutant Y98A | Oryctolagus cuniculus | |
0.0169 | - |
linoleic acid | pH 7.4, 20°, wild-type | Oryctolagus cuniculus | |
0.0213 | - |
linoleic acid | pH 7.4, 20°, mutant Y98F | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
linoleic acid + O2 | - |
Oryctolagus cuniculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
12/15-lipoxygenases | - |
Oryctolagus cuniculus |
12/15-LOX | - |
Oryctolagus cuniculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14.7 | - |
linoleic acid | pH 7.4, 20°, mutant Y98A | Oryctolagus cuniculus | |
44.2 | - |
linoleic acid | pH 7.4, 20°, wild-type | Oryctolagus cuniculus | |
65.2 | - |
linoleic acid | pH 7.4, 20°, mutant Y98F | Oryctolagus cuniculus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1170 | - |
linoleic acid | pH 7.4, 20°, mutant Y98A | Oryctolagus cuniculus | |
2600 | - |
linoleic acid | pH 7.4, 20°, wild-type | Oryctolagus cuniculus | |
3060 | - |
linoleic acid | pH 7.4, 20°, mutant Y98F | Oryctolagus cuniculus |