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Literature summary for 1.13.11.31 extracted from

  • Schurmann, K.; Anton, M.; Ivanov, I.; Richter, C.; Kuhn, H.; Walther, M.
    Molecular basis for the reduced catalytic activity of the naturally occurring T560M mutant of human 12/15-lipoxygenase that has been implicated in coronary artery disease (2011), J. Biol. Chem., 286, 23920-23927.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.31

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Mus musculus
expressed in Escherichia coli as a His-tagged fusion protein Homo sapiens

Protein Variants

Protein Variants Comment Organism
Q294L relative catalytic activity: 16.1% compared to wild-type 100% Homo sapiens
Q303L mutant shows strongly impared catalytic activity, relative catalytic activity: 3.2% compared to wild-type 100% Mus musculus
T560M mutant shows impaired catalytic activity, relative catalytic activity: 25% compared to wild-type 100% Homo sapiens
T570M mutant shows strongly impared catalytic activity, relative catalytic activity: 8.2% compared to wild-type 100% Mus musculus

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Mus musculus
using Ni-NTA chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arachidonic acid + O2
-
 Mus musculus ?
-
 ?
arachidonic acid + O2
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
ALOX12
-
 Homo sapiens
ALOX5
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Mus musculus
25
-
 assay at Homo sapiens