Activating Compound | Comment | Organism | Structure |
---|---|---|---|
13-(S)-hydroxyoctadecadienoic acid | allosteric effector, which changes the conformation of 15-hLO-2 such that substrate affinity toward linoleic acid is significantly increased | Homo sapiens |
Application | Comment | Organism |
---|---|---|
medicine | pro-tumorigenic feedback loop for 13-(S)-hydroxyoctadecadienoic acid against 15-hLO-2 | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
without a His6-tag | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
linoleic acid | fully deuterated, the mode of inhibition, in the presence of 13-(S)-hydroxyoctadecadienoic acid, changes from mixed-type inhibition to competitive inhibition | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | iron-containing metalloenzyme | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
without a His6-tag, more than 90% pure | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epithelium | - |
Homo sapiens | - |
prostate | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonate + O2 | - |
Homo sapiens | (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
15-hLO-2 | - |
Homo sapiens |
15-lipoxygenase-2 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.57 | - |
arachidonic acid | at 15°C, normalized to iron content | Homo sapiens | |
0.62 | - |
arachidonic acid | at pH 7 | Homo sapiens | |
0.74 | - |
arachidonic acid | at pH 8 | Homo sapiens | |
0.75 | - |
arachidonic acid | at pH 7.5 | Homo sapiens | |
0.75 | - |
arachidonic acid | at 22°C, normalized to iron content | Homo sapiens | |
1.04 | - |
arachidonic acid | at 30°C, normalized to iron content | Homo sapiens | |
1.38 | - |
arachidonic acid | at 37°C, normalized to iron content | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | approximately level between pH 7 and 8 | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
linoleic acid | fully deuterated, in the presence of 13-(S)-hydroxyoctadecadienoic acid | Homo sapiens | |
0.017 | - |
linoleic acid | fully deuterated | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00005 | - |
arachidonic acid | at 15°C, normalized to iron content | Homo sapiens | |
0.00015 | - |
arachidonic acid | at 22°C, normalized to iron content | Homo sapiens | |
0.00015 | - |
arachidonic acid | at pH 7.5 | Homo sapiens | |
0.00024 | - |
arachidonic acid | at pH 7 | Homo sapiens | |
0.00028 | - |
arachidonic acid | at pH 8 | Homo sapiens | |
0.00046 | - |
arachidonic acid | at 30°C, normalized to iron content | Homo sapiens | |
0.00075 | - |
arachidonic acid | at 37°C, normalized to iron content | Homo sapiens |