Activating Compound | Comment | Organism | Structure |
---|---|---|---|
5-LO activating protein | i.e. FLAP, Ca2+ induces the translocation of 5-LO from a soluble compartment to nuclear structures, where 5-LO co-localizes with 5-LO activating protein | Homo sapiens | |
ATP | - |
Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of regulatory C2-like domain of the enzyme, residues 1-128, containing a tobacco etch virus protease-cleavage site, in Escherichia coli strain BL21(DE3) as soluble maltose-binding-protein fusion protein | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
methanol | complete inhibition at 50% v/v | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | Ca2+ induces the translocation of 5-LO from a soluble compartment to nuclear structures, where 5-LO co-localizes with 5-LO activating protein, FLAP | Homo sapiens | 5829 | - |
nucleus | Ca2+ induces the translocation of 5-LO from a soluble compartment to nuclear structures, where 5-LO co-localizes with 5-LO activating protein, FLAP | Homo sapiens | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates, Ca2+ induces the translocation of 5-LO from a soluble compartment to nuclear structures, where 5-LO co-localizes with 5-LO activating protein, FLAP | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(6E,8Z,11Z,14Z)-(5S)-5-hydroperoxyeicosa-6,8,11,14-tetraenoate | Homo sapiens | - |
leukotriene A4 + H2O | is unstable and metabolized to leukotriene B4 | ? | |
arachidonic acid + O2 | Homo sapiens | - |
(6E,8Z,11Z,14Z)-(5S)-5-hydroperoxyeicosa-6,8,11,14-tetraenoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | enzyme regulation by phosphorylation | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant soluble MBP-regulatory C2-like domain fusion protein from Escherichia coli strain BL21(DE3) by amylose affinity chromatography, fusion protein cleavage by TEV protease, and further purification of the C2-like domain by hydrophobic interaction chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(6E,8Z,11Z,14Z)-(5S)-5-hydroperoxyeicosa-6,8,11,14-tetraenoate | - |
Homo sapiens | leukotriene A4 + H2O | - |
? | |
(6E,8Z,11Z,14Z)-(5S)-5-hydroperoxyeicosa-6,8,11,14-tetraenoate | - |
Homo sapiens | leukotriene A4 + H2O | is unstable and metabolized to leukotriene B4 | ? | |
arachidonic acid + O2 | - |
Homo sapiens | (6E,8Z,11Z,14Z)-(5S)-5-hydroperoxyeicosa-6,8,11,14-tetraenoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | residues 1-128 of the enzyme form the regulatory C2-like domain | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
5-lipoxygenase | - |
Homo sapiens |
5-LO | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |