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Literature summary for 1.13.11.36 extracted from

  • Muller, R.; Schmitt, S.; Lingens, F.
    A novel non-heme iron-containing dioxygenase. Chloridazon-catechol dioxygenase from Phenylobacterium immobilis DSM 1986 (1982), Eur. J. Biochem., 125, 579-584.
    View publication on PubMed
Search for more literature for 1.13.11.36

Activating Compound

Activating Compound Comment Organism Structure
ascorbate protects against autooxidation Phenylobacterium immobile

Crystallization (Commentary)

Crystallization (Comment) Organism
with 37% ammonium sulfate Phenylobacterium immobile

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required for activity Phenylobacterium immobile

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-amino-4-chloro-2-(2,3-dihydroxyphenyl)-3(2H)-pyridazinone + O2 Phenylobacterium immobile
-
 5-amino-4-chloro-2-(2-hydroxymuconoyl)-3(2H)-pyridazinone
-
 ?

Organism

Organism UniProt Comment Textmining
Phenylobacterium immobile
-
 DSM 1986
-

Oxidation Stability

Oxidation Stability Organism
inactivated by oxygen, can be reactivated by Fe2+ or ascorbate, Fe2+ much more effective in reactivation Phenylobacterium immobile

Storage Stability

Storage Stability Organism
-20°C, stable for months Phenylobacterium immobile
4°C, crystal suspensions stable for several weeks Phenylobacterium immobile

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-amino-4-chloro-2-(2,3-dihydroxyphenyl)-3(2H)-pyridazinone + O2
-
 Phenylobacterium immobile 5-amino-4-chloro-2-(2-hydroxymuconoyl)-3(2H)-pyridazinone
-
 ?
catechol + O2
-
 Phenylobacterium immobile 2-hydroxymuconic acid semialdehyde
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
13.3
-
 catechol
-
 Phenylobacterium immobile