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Literature summary for 1.13.11.39 extracted from

  • Asturias, J.A.; Eltis, L.D.; Prucha, M.; Timmis, K.N.
    Analysis of three 2,3-dihydroxybiphenyl 1,2-dioxygenases found in Rhodococcus globerulus P6. Identification of a new family of extradiol dioxygenases (1994), J. Biol. Chem., 269, 7807-7815.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
nucleotide sequence of bhpB and 3 bphC genes determined, 2 enzymes expressed in Escherichia coli K38 Rhodococcus globerulus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21000
-
6 * 21000 Rhodococcus globerulus
120000
-
-
Rhodococcus globerulus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
biphenyl-2,3-diol + O2 Pseudomonas putida
-
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
?
biphenyl-2,3-diol + O2 Rhodococcus globerulus aerobic biphenyl biodegradation pathway 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
?
biphenyl-2,3-diol + O2 Rhodococcus globerulus P6 aerobic biphenyl biodegradation pathway 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Rhodococcus globerulus
-
-
-
Rhodococcus globerulus P6
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, expressed in Escherichia coli Rhodococcus globerulus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
biphenyl-2,3-diol + O2
-
Pseudomonas putida 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
?
biphenyl-2,3-diol + O2 ring metacleavage Rhodococcus globerulus 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
?
biphenyl-2,3-diol + O2 aerobic biphenyl biodegradation pathway Rhodococcus globerulus 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
?
biphenyl-2,3-diol + O2 ring metacleavage Rhodococcus globerulus P6 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
?
biphenyl-2,3-diol + O2 aerobic biphenyl biodegradation pathway Rhodococcus globerulus P6 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 21000 Rhodococcus globerulus