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Literature summary for 1.13.11.39 extracted from

  • Li, A.; Qu, Y.; Zhou, J.; Ma, F.
    Enzyme-substrate interaction and characterization of a 2,3-dihydroxybiphenyl 1,2-dioxygenase from Dyella ginsengisoli LA-4 (2009), FEMS Microbiol. Lett., 292, 231-239.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
subcloned into the pET28(a) vector, yielding pET-bphC and overexpressed in Escherichia coli BL21 (DE3) Dyella ginsengisoli

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
Dyella ginsengisoli
H2O2
-
Dyella ginsengisoli
KI 600 mM reduces specific activity by 35% Dyella ginsengisoli
L-ascorbic acid completely inhibits at concentrations over 25 mM Dyella ginsengisoli
SDS 1.25 mM inhibits nearly 60% Dyella ginsengisoli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0189
-
2,3-Dihydroxybiphenyl pH 8.0, 20°C Dyella ginsengisoli
0.0496
-
4-Chlorocatechol pH 8.0, 20°C Dyella ginsengisoli
0.0688
-
3-methylcatechol pH 8.0, 20°C Dyella ginsengisoli
0.281
-
catechol pH 8.0, 20°C Dyella ginsengisoli
0.3597
-
4-methylcatechol pH 8.0, 20°C Dyella ginsengisoli

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Dyella ginsengisoli
Fe2+ is an iron (II)-dependent type I extradiol dioxygenase Dyella ginsengisoli
Mg2+
-
Dyella ginsengisoli
additional information Ba2+ has no effect Dyella ginsengisoli

Organism

Organism UniProt Comment Textmining
Dyella ginsengisoli A9QT35 LA-4
-
Dyella ginsengisoli LA-4 A9QT35 LA-4
-

Purification (Commentary)

Purification (Comment) Organism
by gel filtration, more than 95% pure Dyella ginsengisoli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
118.3
-
with 2,3-dihydroxybiphenyl as substrate Dyella ginsengisoli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-dihydroxybiphenyl + O2 best substrate Dyella ginsengisoli 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
?
2,3-dihydroxybiphenyl + O2 best substrate Dyella ginsengisoli LA-4 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
?
3-methylcatechol + O2
-
Dyella ginsengisoli 2-hydroxy-6-oxohepta-2,4-dienoate
-
?
3-methylcatechol + O2
-
Dyella ginsengisoli LA-4 2-hydroxy-6-oxohepta-2,4-dienoate
-
?
4-chlorocatechol + O2
-
Dyella ginsengisoli 4-chloro-2-hydroxy-6-oxohexa-2,4-dienoate
-
?
4-chlorocatechol + O2
-
Dyella ginsengisoli LA-4 4-chloro-2-hydroxy-6-oxohexa-2,4-dienoate
-
?
4-methylcatechol + O2
-
Dyella ginsengisoli 2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate
-
?
4-methylcatechol + O2
-
Dyella ginsengisoli LA-4 2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate
-
?
catechol + O2
-
Dyella ginsengisoli ?
-
?
catechol + O2
-
Dyella ginsengisoli LA-4 ?
-
?

Synonyms

Synonyms Comment Organism
2,3-dihydroxybiphenyl 1,2-dioxygenase
-
Dyella ginsengisoli
BphC
-
Dyella ginsengisoli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Dyella ginsengisoli

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 70 20°C: about 75% of maximal activity, 70°C: about 85% of maximal activity Dyella ginsengisoli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.7
-
4-methylcatechol pH 8.0, 20°C Dyella ginsengisoli
1
-
4-Chlorocatechol pH 8.0, 20°C Dyella ginsengisoli
6.7
-
catechol pH 8.0, 20°C Dyella ginsengisoli
16
-
3-methylcatechol pH 8.0, 20°C Dyella ginsengisoli
84.1
-
2,3-Dihydroxybiphenyl pH 8.0, 20°C Dyella ginsengisoli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Dyella ginsengisoli

pH Range

pH Minimum pH Maximum Comment Organism
7 9 pH 7.0: about 50% of maximal activity, pH 9.0: about 45% of maximal activity Dyella ginsengisoli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2
-
4-methylcatechol pH 8.0, 20°C Dyella ginsengisoli
20
-
4-Chlorocatechol pH 8.0, 20°C Dyella ginsengisoli
24
-
catechol pH 8.0, 20°C Dyella ginsengisoli
233
-
3-methylcatechol pH 8.0, 20°C Dyella ginsengisoli
4450
-
2,3-Dihydroxybiphenyl pH 8.0, 20°C Dyella ginsengisoli