Cloned (Comment) | Organism |
---|---|
subcloned into the pET28(a) vector, yielding pET-bphC and overexpressed in Escherichia coli BL21 (DE3) | Dyella ginsengisoli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Dyella ginsengisoli | |
H2O2 | - |
Dyella ginsengisoli | |
KI | 600 mM reduces specific activity by 35% | Dyella ginsengisoli | |
L-ascorbic acid | completely inhibits at concentrations over 25 mM | Dyella ginsengisoli | |
SDS | 1.25 mM inhibits nearly 60% | Dyella ginsengisoli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0189 | - |
2,3-Dihydroxybiphenyl | pH 8.0, 20°C | Dyella ginsengisoli | |
0.0496 | - |
4-Chlorocatechol | pH 8.0, 20°C | Dyella ginsengisoli | |
0.0688 | - |
3-methylcatechol | pH 8.0, 20°C | Dyella ginsengisoli | |
0.281 | - |
catechol | pH 8.0, 20°C | Dyella ginsengisoli | |
0.3597 | - |
4-methylcatechol | pH 8.0, 20°C | Dyella ginsengisoli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Dyella ginsengisoli | |
Fe2+ | is an iron (II)-dependent type I extradiol dioxygenase | Dyella ginsengisoli | |
Mg2+ | - |
Dyella ginsengisoli | |
additional information | Ba2+ has no effect | Dyella ginsengisoli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dyella ginsengisoli | A9QT35 | LA-4 | - |
Dyella ginsengisoli LA-4 | A9QT35 | LA-4 | - |
Purification (Comment) | Organism |
---|---|
by gel filtration, more than 95% pure | Dyella ginsengisoli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
118.3 | - |
with 2,3-dihydroxybiphenyl as substrate | Dyella ginsengisoli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-dihydroxybiphenyl + O2 | best substrate | Dyella ginsengisoli | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O | - |
? | |
2,3-dihydroxybiphenyl + O2 | best substrate | Dyella ginsengisoli LA-4 | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O | - |
? | |
3-methylcatechol + O2 | - |
Dyella ginsengisoli | 2-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
3-methylcatechol + O2 | - |
Dyella ginsengisoli LA-4 | 2-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
4-chlorocatechol + O2 | - |
Dyella ginsengisoli | 4-chloro-2-hydroxy-6-oxohexa-2,4-dienoate | - |
? | |
4-chlorocatechol + O2 | - |
Dyella ginsengisoli LA-4 | 4-chloro-2-hydroxy-6-oxohexa-2,4-dienoate | - |
? | |
4-methylcatechol + O2 | - |
Dyella ginsengisoli | 2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate | - |
? | |
4-methylcatechol + O2 | - |
Dyella ginsengisoli LA-4 | 2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate | - |
? | |
catechol + O2 | - |
Dyella ginsengisoli | ? | - |
? | |
catechol + O2 | - |
Dyella ginsengisoli LA-4 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2,3-dihydroxybiphenyl 1,2-dioxygenase | - |
Dyella ginsengisoli |
BphC | - |
Dyella ginsengisoli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Dyella ginsengisoli |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | 20°C: about 75% of maximal activity, 70°C: about 85% of maximal activity | Dyella ginsengisoli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
4-methylcatechol | pH 8.0, 20°C | Dyella ginsengisoli | |
1 | - |
4-Chlorocatechol | pH 8.0, 20°C | Dyella ginsengisoli | |
6.7 | - |
catechol | pH 8.0, 20°C | Dyella ginsengisoli | |
16 | - |
3-methylcatechol | pH 8.0, 20°C | Dyella ginsengisoli | |
84.1 | - |
2,3-Dihydroxybiphenyl | pH 8.0, 20°C | Dyella ginsengisoli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Dyella ginsengisoli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | pH 7.0: about 50% of maximal activity, pH 9.0: about 45% of maximal activity | Dyella ginsengisoli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
4-methylcatechol | pH 8.0, 20°C | Dyella ginsengisoli | |
20 | - |
4-Chlorocatechol | pH 8.0, 20°C | Dyella ginsengisoli | |
24 | - |
catechol | pH 8.0, 20°C | Dyella ginsengisoli | |
233 | - |
3-methylcatechol | pH 8.0, 20°C | Dyella ginsengisoli | |
4450 | - |
2,3-Dihydroxybiphenyl | pH 8.0, 20°C | Dyella ginsengisoli |