Literature summary for 1.13.11.39 extracted from

Senda, M.; Hatta, T.; Kimbara, K.; Senda, T.
Crystallization and preliminary crystallographic analysis of manganese(II)-dependent 2,3-dihydroxybiphenyl 1,2-dioxygenase from Bacillus sp. JF8 (2010), Acta Crystallogr. Sect. F, 66, 282-285.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli MV1190 cells	Bacillus sp. (in: Bacteria)

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 30% (v/v) PEG 400, 200 mM magnesium chloride, 100 mM Tris-HCl pH 8.5 or 10% (v/v) 2-propanol, 100 mM MES-NaOH pH 6.5 and 200 mM zinc acetate	Bacillus sp. (in: Bacteria)

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	dependent on	Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
36300	-	4 * 36300, X-ray crystallography	Bacillus sp. (in: Bacteria)

Organism

Organism	UniProt	Comment	Textmining
Bacillus sp. (in: Bacteria)	-	-	-
Bacillus sp. (in: Bacteria) JF8	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
DEAE Toyopearl column chromatography, phenyl Sepharose column chromatography, and Mono Q column chromatography	Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
biphenyl-2,3-diol + O2	-	Bacillus sp. (in: Bacteria)	2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O	-	?
biphenyl-2,3-diol + O2	-	Bacillus sp. (in: Bacteria) JF8	2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O	-	?

Subunits

Subunits	Comment	Organism
homotetramer	4 * 36300, X-ray crystallography	Bacillus sp. (in: Bacteria)

Synonyms

Synonyms	Comment	Organism
BphC	-	Bacillus sp. (in: Bacteria)
manganese(II)-dependent 2,3-dihydroxybiphenyl 1,2-dioxygenase	-	Bacillus sp. (in: Bacteria)

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Release 2023.1 (January 2023)