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Literature summary for 1.13.11.39 extracted from

  • Gou, M.; Qu, Y.; Xu, B.; Zhou, J.; Li, X.; Zhou, H.
    Isolation, characterization and docking studies of 2,3-dihydroxybiphenyl 1,2-dioxygenase from an activated sludge metagenome (2012), Biotechnol. Lett., 34, 117-123.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene bphC_meta, DNA and amino acid sequence determination and analysis, expression in Escherichia coli uncultured bacterium

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 1 mM, moderate inhibition uncultured bacterium
Ca2+ 1 mM, slight inhibition with substrate biphenyl-2,3-diol, moderate with catechol, but activating with 3-methylcatechol uncultured bacterium
Co2+ 1 mM, complete inhibition uncultured bacterium
Cu2+ 1 mM, complete inhibition uncultured bacterium
Hg2+ 1 mM, complete inhibition uncultured bacterium
Mg2+ 1 mM, moderate inhibition uncultured bacterium
Mn2+ 1 mM, moderate inhibition uncultured bacterium
Ni2+ 1 mM, strong inhibition uncultured bacterium
Zn2+ 1 mM, complete inhibition with substrate biphenyl-2,3-diol, not with catechols uncultured bacterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0067
-
biphenyl-2,3-diol pH 9.0, 40°C, recombinant enzyme uncultured bacterium
0.49
-
3-methylcatechol pH 9.0, 40°C, recombinant enzyme uncultured bacterium
0.606
-
catechol pH 9.0, 40°C, recombinant enzyme uncultured bacterium

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM, slight inhibition with substrate biphenyl-2,3-diol, moderate with catechol, but activating with 3-methylcatechol uncultured bacterium
Fe3+ 1 mM, activates uncultured bacterium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
x * 32000, recombinant enzyme, SDS-PAGE uncultured bacterium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
biphenyl-2,3-diol + O2 uncultured bacterium
-
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
-
?

Organism

Organism UniProt Comment Textmining
uncultured bacterium F2X5L9 activated sludge metagenome, gene bphC_meta
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli uncultured bacterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methylcatechol + O2
-
uncultured bacterium ?
-
?
biphenyl-2,3-diol + O2
-
uncultured bacterium 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
-
?
biphenyl-2,3-diol + O2 preferred substrate uncultured bacterium 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
-
?
catechol + O2
-
uncultured bacterium cis,cis-muconate
-
?
additional information activity of recombinant enzyme toward substituted catechols is in descending order: 2,3-dihydroxybiphenyl, 3-methylcatechol, catechol, and 4-chlorocatechol/4-methylcatechol, substrate binding analysis by molecular docking uncultured bacterium ?
-
?

Subunits

Subunits Comment Organism
? x * 32000, recombinant enzyme, SDS-PAGE uncultured bacterium
More enzyme homology modeling, overview uncultured bacterium

Synonyms

Synonyms Comment Organism
2,3-dihydroxybiphenyl 1,2-dioxygenase
-
uncultured bacterium
BphC
-
uncultured bacterium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
recombinant enzyme uncultured bacterium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
38
-
catechol pH 9.0, 40°C, recombinant enzyme uncultured bacterium
44
-
3-methylcatechol pH 9.0, 40°C, recombinant enzyme uncultured bacterium
165
-
biphenyl-2,3-diol pH 9.0, 40°C, recombinant enzyme uncultured bacterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
recombinant enzyme uncultured bacterium

General Information

General Information Comment Organism
metabolism BphC is the key enzyme for polychlorobiphenyl transformation uncultured bacterium
additional information enzyme homology modeling, overview uncultured bacterium
physiological function BphC catalyzes the ring cleavage of 2,3-dihydroxybiphenyl during biphenyl/polychlorobipheny degradation process uncultured bacterium

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
62.7
-
catechol pH 9.0, 40°C, recombinant enzyme uncultured bacterium
89.8
-
3-methylcatechol pH 9.0, 40°C, recombinant enzyme uncultured bacterium
24630
-
biphenyl-2,3-diol pH 9.0, 40°C, recombinant enzyme uncultured bacterium