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Literature summary for 1.13.11.4 extracted from

  • Yeo, C.C.; Tan, C.L.; Gao, X.; Zhao, B.; Poh, C.L.
    Characterization of hbzE-encoded gentisate 1,2-dioxygenase from Pseudomonas alcaligenes NCIMB 9867 (2007), Res. Microbiol., 158, 608-616.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed as a hexahistidine fusion protein from Escherichia coli Pseudomonas alcaligenes

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 5 mM, partial inhibition Pseudomonas alcaligenes
Cu2+ 5 mM, complete inhibition Pseudomonas alcaligenes
EDTA 5 mM, more than 95% inhibition Pseudomonas alcaligenes
Fe2+ 1 mM, complete inactivation Pseudomonas alcaligenes
Hg2+ 5 mM, more than 95% inhibition Pseudomonas alcaligenes
Mn2+ 5 mM, partial inhibition Pseudomonas alcaligenes
Na+ 5 mM, partial inhibition Pseudomonas alcaligenes
Zn2+ 5 mM, more than 95% inhibition Pseudomonas alcaligenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01155
-
3-Ethylgentisate pH 7.4, 23°C Pseudomonas alcaligenes
0.029
-
3-Bromogentisate pH 7.4, 23°C Pseudomonas alcaligenes
0.0313
-
3-Isopropylgentisate pH 7.4, 23°C Pseudomonas alcaligenes
0.0505
-
3-Fluorogentisate pH 7.4, 23°C Pseudomonas alcaligenes
0.0549
-
3-Methylgentisate pH 7.4, 23°C Pseudomonas alcaligenes
0.113
-
2,5-Dihydroxybenzoate pH 7.4, 23°C Pseudomonas alcaligenes

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 0.05-0.1 mM, 160% increase in activity Pseudomonas alcaligenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
x * 40000, SDS-PAGE Pseudomonas alcaligenes
150000
-
non-denaturing PAGE Pseudomonas alcaligenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas alcaligenes GDO-II is strictly inducible by aromatic substrates such as 3-hydroxybenzoate and gentisate ?
-
?
additional information Pseudomonas alcaligenes P25X GDO-II is strictly inducible by aromatic substrates such as 3-hydroxybenzoate and gentisate ?
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas alcaligenes
-
-
-
Pseudomonas alcaligenes P25X
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Pseudomonas alcaligenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,5-dihydroxybenzoate + O2 i.e. gentisate Pseudomonas alcaligenes maleylpyruvate
-
?
2,5-dihydroxybenzoate + O2 i.e. gentisate Pseudomonas alcaligenes P25X maleylpyruvate
-
?
3-bromogentisate + O2 Vmax is 48.5% of Vmax for gentisate Pseudomonas alcaligenes 3-bromo-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
?
3-ethylgentisate + O2 Vmax is 39.7% of Vmax for gentisate Pseudomonas alcaligenes 3-ethyl-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
?
3-ethylgentisate + O2 Vmax is 39.7% of Vmax for gentisate Pseudomonas alcaligenes P25X 3-ethyl-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
?
3-fluorogentisate + O2 Vmax of recombinant enzyme is 20.5% higher compared to gentisate Pseudomonas alcaligenes 3-fluoro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
?
3-fluorogentisate + O2 Vmax of recombinant enzyme is 20.5% higher compared to gentisate Pseudomonas alcaligenes P25X 3-fluoro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
?
3-isopropylgentisate + O2 Vmax is 57.1% of Vmax for gentisate Pseudomonas alcaligenes 4-hydroxy-3-isopropyl-6-oxo-hepta-2,4-dienedioic acid
-
?
3-methylgentisate + O2 Vmax is 10.1% of Vmax for gentisate Pseudomonas alcaligenes 3-methyl-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
?
additional information GDO-II is strictly inducible by aromatic substrates such as 3-hydroxybenzoate and gentisate Pseudomonas alcaligenes ?
-
?
additional information GDO-II is unable to utilize gentisate that is substituted at the carbon-4 position (i.e., 4-chloro- and 4-methylgentisate) Pseudomonas alcaligenes ?
-
?
additional information GDO-II is strictly inducible by aromatic substrates such as 3-hydroxybenzoate and gentisate Pseudomonas alcaligenes P25X ?
-
?
additional information GDO-II is unable to utilize gentisate that is substituted at the carbon-4 position (i.e., 4-chloro- and 4-methylgentisate) Pseudomonas alcaligenes P25X ?
-
?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Pseudomonas alcaligenes

Synonyms

Synonyms Comment Organism
GDO-II
-
Pseudomonas alcaligenes
hbzE
-
Pseudomonas alcaligenes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
Pseudomonas alcaligenes

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15
-
no activity below Pseudomonas alcaligenes
60
-
no activity above Pseudomonas alcaligenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
Pseudomonas alcaligenes

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas alcaligenes pH range 3.5-9.5 4.7 4.5