Cloned (Comment) | Organism |
---|---|
overexpressed as a hexahistidine fusion protein from Escherichia coli | Pseudomonas alcaligenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 5 mM, partial inhibition | Pseudomonas alcaligenes | |
Cu2+ | 5 mM, complete inhibition | Pseudomonas alcaligenes | |
EDTA | 5 mM, more than 95% inhibition | Pseudomonas alcaligenes | |
Fe2+ | 1 mM, complete inactivation | Pseudomonas alcaligenes | |
Hg2+ | 5 mM, more than 95% inhibition | Pseudomonas alcaligenes | |
Mn2+ | 5 mM, partial inhibition | Pseudomonas alcaligenes | |
Na+ | 5 mM, partial inhibition | Pseudomonas alcaligenes | |
Zn2+ | 5 mM, more than 95% inhibition | Pseudomonas alcaligenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01155 | - |
3-Ethylgentisate | pH 7.4, 23°C | Pseudomonas alcaligenes | |
0.029 | - |
3-Bromogentisate | pH 7.4, 23°C | Pseudomonas alcaligenes | |
0.0313 | - |
3-Isopropylgentisate | pH 7.4, 23°C | Pseudomonas alcaligenes | |
0.0505 | - |
3-Fluorogentisate | pH 7.4, 23°C | Pseudomonas alcaligenes | |
0.0549 | - |
3-Methylgentisate | pH 7.4, 23°C | Pseudomonas alcaligenes | |
0.113 | - |
2,5-Dihydroxybenzoate | pH 7.4, 23°C | Pseudomonas alcaligenes |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 0.05-0.1 mM, 160% increase in activity | Pseudomonas alcaligenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, SDS-PAGE | Pseudomonas alcaligenes |
150000 | - |
non-denaturing PAGE | Pseudomonas alcaligenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudomonas alcaligenes | GDO-II is strictly inducible by aromatic substrates such as 3-hydroxybenzoate and gentisate | ? | - |
? | |
additional information | Pseudomonas alcaligenes P25X | GDO-II is strictly inducible by aromatic substrates such as 3-hydroxybenzoate and gentisate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas alcaligenes | - |
- |
- |
Pseudomonas alcaligenes P25X | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Pseudomonas alcaligenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-dihydroxybenzoate + O2 | i.e. gentisate | Pseudomonas alcaligenes | maleylpyruvate | - |
? | |
2,5-dihydroxybenzoate + O2 | i.e. gentisate | Pseudomonas alcaligenes P25X | maleylpyruvate | - |
? | |
3-bromogentisate + O2 | Vmax is 48.5% of Vmax for gentisate | Pseudomonas alcaligenes | 3-bromo-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
3-ethylgentisate + O2 | Vmax is 39.7% of Vmax for gentisate | Pseudomonas alcaligenes | 3-ethyl-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
3-ethylgentisate + O2 | Vmax is 39.7% of Vmax for gentisate | Pseudomonas alcaligenes P25X | 3-ethyl-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
3-fluorogentisate + O2 | Vmax of recombinant enzyme is 20.5% higher compared to gentisate | Pseudomonas alcaligenes | 3-fluoro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
3-fluorogentisate + O2 | Vmax of recombinant enzyme is 20.5% higher compared to gentisate | Pseudomonas alcaligenes P25X | 3-fluoro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
3-isopropylgentisate + O2 | Vmax is 57.1% of Vmax for gentisate | Pseudomonas alcaligenes | 4-hydroxy-3-isopropyl-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
3-methylgentisate + O2 | Vmax is 10.1% of Vmax for gentisate | Pseudomonas alcaligenes | 3-methyl-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
additional information | GDO-II is strictly inducible by aromatic substrates such as 3-hydroxybenzoate and gentisate | Pseudomonas alcaligenes | ? | - |
? | |
additional information | GDO-II is unable to utilize gentisate that is substituted at the carbon-4 position (i.e., 4-chloro- and 4-methylgentisate) | Pseudomonas alcaligenes | ? | - |
? | |
additional information | GDO-II is strictly inducible by aromatic substrates such as 3-hydroxybenzoate and gentisate | Pseudomonas alcaligenes P25X | ? | - |
? | |
additional information | GDO-II is unable to utilize gentisate that is substituted at the carbon-4 position (i.e., 4-chloro- and 4-methylgentisate) | Pseudomonas alcaligenes P25X | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE | Pseudomonas alcaligenes |
Synonyms | Comment | Organism |
---|---|---|
GDO-II | - |
Pseudomonas alcaligenes |
hbzE | - |
Pseudomonas alcaligenes |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Pseudomonas alcaligenes |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | - |
no activity below | Pseudomonas alcaligenes |
60 | - |
no activity above | Pseudomonas alcaligenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Pseudomonas alcaligenes |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas alcaligenes | pH range 3.5-9.5 | 4.7 | 4.5 |