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Literature summary for 1.13.11.45 extracted from
- Manganese lipoxygenase. Purification and characterization (1998), J. Biol. Chem., 273, 13072-13079.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| arachidonic acid | inhibits at 0.01 mM | Gaeumannomyces graminis |  |
| eicosatetraynoic acid | 50% of control activity at 0.01 mM | Gaeumannomyces graminis | |
| additional information | no inhibition by lipoxygenase inhibitors zileuton, esculetin and ICI 230487 | Gaeumannomyces graminis | |
| N-(3-phenoxycinnamyl)acetohydroxamic acid | 50% of control activity at 0.05 mM | Gaeumannomyces graminis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0024 | - |
alpha-linolenic acid | - |
Gaeumannomyces graminis | |
| 0.0044 | - |
linoleic acid | - |
Gaeumannomyces graminis | |
| 0.031 | - |
O2 | - |
Gaeumannomyces graminis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | contains 0.5-1.0 atom per enzyme molecule | Gaeumannomyces graminis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 73000 | - |
SDS-PAGE after deglycosylation | Gaeumannomyces graminis |
| 135000 | - |
gel filtration with Superdex 200 in 6 M urea | Gaeumannomyces graminis |
| 160000 | - |
gel filtration with Superdex 200 | Gaeumannomyces graminis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gaeumannomyces graminis | - |
take-all fungus | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Gaeumannomyces graminis |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Gaeumannomyces graminis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 3 mM NaN3, 0.2 mM GSH, several months, no loss of activity | Gaeumannomyces graminis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-linolenic acid + O2 | - |
Gaeumannomyces graminis | (9Z,11E,15Z)-(13)-hydroperoxyoctadecatrienoic acid | - |
? | |
| gamma-linolenic acid + O2 | - |
Gaeumannomyces graminis | (6Z,9Z,11E)-(13)-hydroperoxyoctadecatrienoic acid + (6Z,9Z,12Z)-(11)-hydroperoxyoctadecatrienoic acid | - |
? | |
| linoleic acid + O2 | - |
Gaeumannomyces graminis | (9Z,11E)-(13R)-hydroperoxyoctadecadienoic acid | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Gaeumannomyces graminis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 12 | 60 | 8.8% of maximal activity at 12°C and 100% of maximal activity at 60°C, activity decreases sharply at 63°C | Gaeumannomyces graminis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 63 | - |
incubation for 5 min over 63°C decreases activity, no activity at 75°C | Gaeumannomyces graminis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 18.3 | - |
linoleic acid | - |
Gaeumannomyces graminis | |
| 23.3 | - |
O2 | - |
Gaeumannomyces graminis | |
| 40 | - |
alpha-linolenic acid | - |
Gaeumannomyces graminis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
broad pH optimum | Gaeumannomyces graminis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 11 | at pH 5.0 64% of maximum activity, at pH 11 69% of maximum activity | Gaeumannomyces graminis |
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