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Literature summary for 1.13.11.49 extracted from
- Chemical and steady-state kinetic analyses of a heterologously expressed heme dependent chlorite dismutase (2008), Biochemistry, 47, 5271-5280.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| chloride | mixed inhibitor | Dechloromonas agitata |  |
| Chlorite | irreversibly inactivates the enzyme after 17000 turnovers (per heme) and with a half-life of 0.39 min, resulting in bleaching of the heme chromophore. Guaiacol offers incomplete protection of the enzyme from inactivation | Dechloromonas agitata | |
| additional information | O2 is not an inhibitor | Dechloromonas agitata |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.215 | - |
Chlorite | - |
Dechloromonas agitata | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28400 | - |
4 * 28400, SDS-PAGE | Dechloromonas agitata |
| 116000 | - |
gel filtration | Dechloromonas agitata |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dechloromonas agitata | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Dechloromonas agitata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chlorite | - |
Dechloromonas agitata | chloride + O2 | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 28400, SDS-PAGE | Dechloromonas agitata |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| chlorite dismutase | - |
Dechloromonas agitata |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7550 | - |
Chlorite | - |
Dechloromonas agitata | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme b | contains 3.7 heme per tetramer | Dechloromonas agitata | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.166 | - |
Chlorite | - |
Dechloromonas agitata | |
| 225 | - |
chloride | - |
Dechloromonas agitata | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Dechloromonas agitata | isoelectric focusing | - |
9.6 |
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