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Literature summary for 1.13.11.5 extracted from

  • Sugumaran, M.; Vaidyanathan, C.S.
    Affinity chromatography of homogentisate-1,2-dioxygenase from Aspergillus niger (1978), FEMS Microbiol. Lett., 4, 343-347.
No PubMed abstract available
Search for more literature for 1.13.11.5

Inhibitors

Inhibitors Comment Organism Structure
2-hydroxyphenylacetate strong competitive inhibition with respect to homogentisate Aspergillus niger
3-hydroxyphenylacetate competitive inhibition with respect to homogentisate Aspergillus niger
sulfhydryl reagents strong inhibition Aspergillus niger
Superoxide dismutase inhibits the reaction indicating the involvement of superoxide anions in the dioxygenase reaction Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6
-
 homogentisate
-
 Aspergillus niger

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ requires Fe2+ Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
200000
-
 gel filtration Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
homogentisate + O2 Aspergillus niger one of the key enzymes involved in catabolism of phenylalanine, tyrosine, phenylacetic acid and hydroxyphenylacetic acids 4-maleylacetoacetate
-
 ?
homogentisate + O2 Aspergillus niger UBC 814 one of the key enzymes involved in catabolism of phenylalanine, tyrosine, phenylacetic acid and hydroxyphenylacetic acids 4-maleylacetoacetate
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
 UBC 814
-
Aspergillus niger UBC 814
-
 UBC 814
-

Purification (Commentary)

Purification (Comment) Organism
19.2fold purification Aspergillus niger

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.17
-
-
 Aspergillus niger

Storage Stability

Storage Stability Organism
-20°C, in absence of Fe2+, about 3 days, stable Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
homogentisate + O2 specific for homogentisate Aspergillus niger 4-maleylacetoacetate
-
 ?
homogentisate + O2 one of the key enzymes involved in catabolism of phenylalanine, tyrosine, phenylacetic acid and hydroxyphenylacetic acids Aspergillus niger 4-maleylacetoacetate
-
 ?
homogentisate + O2 specific for homogentisate Aspergillus niger UBC 814 4-maleylacetoacetate
-
 ?
homogentisate + O2 one of the key enzymes involved in catabolism of phenylalanine, tyrosine, phenylacetic acid and hydroxyphenylacetic acids Aspergillus niger UBC 814 4-maleylacetoacetate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4
-
-
 Aspergillus niger