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Literature summary for 1.13.11.50 extracted from

  • Straganz, G.D.; Diebold, A.R.; Egger, S.; Nidetzky, B.; Solomon, E.I.
    Kinetic and CD/MCD spectroscopic studies of the atypical, three-His-ligated, non-heme Fe2+ center in diketone dioxygenase: the role of hydrophilic outer shell residues in catalysis (2010), Biochemistry, 49, 996-1004.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
E98A site-direted mutagenesis Acinetobacter johnsonii
E98Q site-direted mutagenesis Acinetobacter johnsonii
R80A site-direted mutagenesis Acinetobacter johnsonii
R80A/E98A site-direted mutagenesis Acinetobacter johnsonii
T107A site-direted mutagenesis Acinetobacter johnsonii
Y70A site-direted mutagenesis Acinetobacter johnsonii
Y70A/R80A/E98A site-direted mutagenesis Acinetobacter johnsonii
Y70F site-direted mutagenesis Acinetobacter johnsonii
Y70F/R80A/E98A site-direted mutagenesis Acinetobacter johnsonii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic first-order rate constants of substrate ligation and steady-state kinetic studies of wild-type and mutant enzymes, overview Acinetobacter johnsonii

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ Dke1 contains an atypical, three-histidine-ligated, mononuclear non-heme Fe2+ center, spectroscopic analysis, overview. Stabilizing effect of Glu98 on the 6C geometry of the metal center, priming it for substrate ligation. Also Thr107 stabilizes the Fe(II) cofactor Acinetobacter johnsonii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pentane-2,4-dione + O2 Acinetobacter johnsonii
-
acetate + 2-oxopropanal
-
?

Organism

Organism UniProt Comment Textmining
Acinetobacter johnsonii Q8GNT2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,1,1-trifluoro-pentane-2,4-dione + O2
-
Acinetobacter johnsonii ?
-
?
additional information residues Arg80 and Tyr70 promote O2 reduction Acinetobacter johnsonii ?
-
?
pentane-2,4-dione + O2
-
Acinetobacter johnsonii acetate + 2-oxopropanal
-
?

Synonyms

Synonyms Comment Organism
diketone cleaving enzyme
-
Acinetobacter johnsonii
diketone dioxygenase
-
Acinetobacter johnsonii
Dke1
-
Acinetobacter johnsonii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Acinetobacter johnsonii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.007
-
1,1,1-trifluoro-pentane-2,4-dione pH 7.5, 25°C, recombinant mutant Y70A/R80A/E98A Acinetobacter johnsonii
0.033
-
1,1,1-trifluoro-pentane-2,4-dione pH 7.5, 25°C, recombinant mutant Y70F/R80A/E98A Acinetobacter johnsonii
0.05
-
1,1,1-trifluoro-pentane-2,4-dione pH 7.5, 25°C, recombinant mutant E98Q Acinetobacter johnsonii
0.074
-
1,1,1-trifluoro-pentane-2,4-dione pH 7.5, 25°C, recombinant mutant E98A Acinetobacter johnsonii
0.098
-
1,1,1-trifluoro-pentane-2,4-dione pH 7.5, 25°C, recombinant mutant R80A/E98A Acinetobacter johnsonii
0.1
-
1,1,1-trifluoro-pentane-2,4-dione pH 7.5, 25°C, recombinant mutant T107A Acinetobacter johnsonii
0.126
-
1,1,1-trifluoro-pentane-2,4-dione pH 7.5, 25°C, recombinant mutant R80A Acinetobacter johnsonii
0.13
-
1,1,1-trifluoro-pentane-2,4-dione pH 7.5, 25°C, recombinant mutant Y70A Acinetobacter johnsonii
1.27
-
1,1,1-trifluoro-pentane-2,4-dione pH 7.5, 25°C, recombinant mutant Y70F Acinetobacter johnsonii
6.6
-
1,1,1-trifluoro-pentane-2,4-dione pH 7.5, 25°C, recombinant wild-type enzyme Acinetobacter johnsonii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Acinetobacter johnsonii

pH Range

pH Minimum pH Maximum Comment Organism
5 10.5
-
Acinetobacter johnsonii