Protein Variants | Comment | Organism |
---|---|---|
E98A | site-direted mutagenesis | Acinetobacter johnsonii |
E98Q | site-direted mutagenesis | Acinetobacter johnsonii |
R80A | site-direted mutagenesis | Acinetobacter johnsonii |
R80A/E98A | site-direted mutagenesis | Acinetobacter johnsonii |
T107A | site-direted mutagenesis | Acinetobacter johnsonii |
Y70A | site-direted mutagenesis | Acinetobacter johnsonii |
Y70A/R80A/E98A | site-direted mutagenesis | Acinetobacter johnsonii |
Y70F | site-direted mutagenesis | Acinetobacter johnsonii |
Y70F/R80A/E98A | site-direted mutagenesis | Acinetobacter johnsonii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic first-order rate constants of substrate ligation and steady-state kinetic studies of wild-type and mutant enzymes, overview | Acinetobacter johnsonii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | Dke1 contains an atypical, three-histidine-ligated, mononuclear non-heme Fe2+ center, spectroscopic analysis, overview. Stabilizing effect of Glu98 on the 6C geometry of the metal center, priming it for substrate ligation. Also Thr107 stabilizes the Fe(II) cofactor | Acinetobacter johnsonii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pentane-2,4-dione + O2 | Acinetobacter johnsonii | - |
acetate + 2-oxopropanal | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter johnsonii | Q8GNT2 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,1,1-trifluoro-pentane-2,4-dione + O2 | - |
Acinetobacter johnsonii | ? | - |
? | |
additional information | residues Arg80 and Tyr70 promote O2 reduction | Acinetobacter johnsonii | ? | - |
? | |
pentane-2,4-dione + O2 | - |
Acinetobacter johnsonii | acetate + 2-oxopropanal | - |
? |
Synonyms | Comment | Organism |
---|---|---|
diketone cleaving enzyme | - |
Acinetobacter johnsonii |
diketone dioxygenase | - |
Acinetobacter johnsonii |
Dke1 | - |
Acinetobacter johnsonii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Acinetobacter johnsonii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
1,1,1-trifluoro-pentane-2,4-dione | pH 7.5, 25°C, recombinant mutant Y70A/R80A/E98A | Acinetobacter johnsonii | |
0.033 | - |
1,1,1-trifluoro-pentane-2,4-dione | pH 7.5, 25°C, recombinant mutant Y70F/R80A/E98A | Acinetobacter johnsonii | |
0.05 | - |
1,1,1-trifluoro-pentane-2,4-dione | pH 7.5, 25°C, recombinant mutant E98Q | Acinetobacter johnsonii | |
0.074 | - |
1,1,1-trifluoro-pentane-2,4-dione | pH 7.5, 25°C, recombinant mutant E98A | Acinetobacter johnsonii | |
0.098 | - |
1,1,1-trifluoro-pentane-2,4-dione | pH 7.5, 25°C, recombinant mutant R80A/E98A | Acinetobacter johnsonii | |
0.1 | - |
1,1,1-trifluoro-pentane-2,4-dione | pH 7.5, 25°C, recombinant mutant T107A | Acinetobacter johnsonii | |
0.126 | - |
1,1,1-trifluoro-pentane-2,4-dione | pH 7.5, 25°C, recombinant mutant R80A | Acinetobacter johnsonii | |
0.13 | - |
1,1,1-trifluoro-pentane-2,4-dione | pH 7.5, 25°C, recombinant mutant Y70A | Acinetobacter johnsonii | |
1.27 | - |
1,1,1-trifluoro-pentane-2,4-dione | pH 7.5, 25°C, recombinant mutant Y70F | Acinetobacter johnsonii | |
6.6 | - |
1,1,1-trifluoro-pentane-2,4-dione | pH 7.5, 25°C, recombinant wild-type enzyme | Acinetobacter johnsonii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Acinetobacter johnsonii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10.5 | - |
Acinetobacter johnsonii |