Literature summary for 1.13.11.52 extracted from

Takikawa, O.; Littlejohn, T.K.; Truscott, R.J.W.
Indoleamine 2,3-dioxygenase in the human lens, the first enzyme in the synthesis of UV filters (2001), Exp. Eye Res., 72, 271-277.

Search for more literature for 1.13.11.52

Application

Application	Comment	Organism
medicine	first and probably rate-limiting enzyme in UV filter biosynthesis	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
additional information	expressed in epithelial cells but not in mature fiber cells	Homo sapiens	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
45000	-	SDS-PAGE	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-tryptophan + O2	Homo sapiens	-	N-formyl-L-kynurenine	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
0-5°C, homogenization, Protein G-Sepharose	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
lens cortex	anterior and bow region	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	0.0142 nmol/mg/lens, assay in 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.01 mM methylene blue, 0.1 mg/ml catalase, 0.4 mM L-tryptophan, 90 min, 37°C	Homo sapiens
0.072	-	assuming an average wet weight of the lens of 0.2 g, assay in 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.01 mM methylene blue, 0.1 mg/ml catalase, 0.4 mM L-tryptophan, 90 min, 37°C	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-tryptophan + O2	-	Homo sapiens	N-formyl-L-kynurenine	-	?

Synonyms

Synonyms	Comment	Organism
IDO	-	Homo sapiens

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Release 2023.1 (January 2023)