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Literature summary for 1.13.11.52 extracted from
- Human placental indoleamine 2,3-dioxygenase: cellular localization and characterization of an enzyme preventing fetal rejection (2000), Biochim. Biophys. Acta, 1500, 119-124.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-methyl-DL-tryptophan | competitive inhibition with L-tryptophan, 2 mM, 70% inhibition | Homo sapiens |  |
| 1-Methyltryptophan | 2 mM, 70% inhibition; competitive | Homo sapiens | |
| additional information | not inhibited by indo-3-acetamide, beta-indoylacetonitrile snd 3-beta-indoleacrylic acid | Homo sapiens | |
| norharman | 2 mM, 98% inhibition, uncompetitive; uncompetitive inhibition, 2 mM, 98% inhibition | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0246 | - |
L-tryptophan | - |
Homo sapiens | |
| 0.0246 | - |
L-Trp | - |
Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Homo sapiens | 5737 | - |
| cytosol | - |
Homo sapiens | 5829 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| homogenization, filtered through cotton gauze, centrifugation, collection, stored at -20°C | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | placental enzyme is not expressed in the maternal facing brush border membrane of syncytiotrophoblast | Homo sapiens | - |
| placenta | - |
Homo sapiens | - |
| placenta | brush border microvillous plasma membranes of placental syncytiotrophoblast | Homo sapiens | - |
| syncytiotrophoblast | brush border microvillous plasma membranes of placental syncytiotrophoblast | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.000598 | - |
supernatant, 0.8 mM L-tryptophan, 40 mM ascorbic acid, 0.02 mM methylene blue, 200 units/ml catalase, 100 mM potassium phosphate buffer, pH 6.5, 37°C, 30 min | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-hydroxy-L-tryptophan + O2 | - |
Homo sapiens | ? | - |
? | |
| 5-hydroxytryptamine + O2 | - |
Homo sapiens | ? | - |
? | |
| D-Trp + O2 | - |
Homo sapiens | N-formyl-D-kynurenine | - |
? | |
| D-tryptophan + O2 | - |
Homo sapiens | N-formyl-D-kynurenine | - |
? | |
| L-5-hydroxytryptophan + O2 | - |
Homo sapiens | ? | - |
? | |
| L-Trp + O2 | - |
Homo sapiens | L-formylkynurenine | - |
? | |
| L-tryptophan + O2 | - |
Homo sapiens | N-formyl-L-kynurenine | - |
? | |
| tryptamine + O2 | - |
Homo sapiens | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Homo sapiens |
| 6.5 | - |
L-tryptophan | Homo sapiens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.068 | - |
1-Methyltryptophan | - |
Homo sapiens | |
| 0.068 | - |
1-methyl-DL-tryptophan | - |
Homo sapiens | |
| 0.176 | - |
norharman | - |
Homo sapiens | |
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