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Literature summary for 1.13.11.52 extracted from

  • Yamamoto, S.; Hayaishi, O.
    Tryptophan pyrrolase of rabbit intestine (1967), J. Biol. Chem., 242 (22), 5260-5266.
No PubMed abstract available
Search for more literature for 1.13.11.52

Inhibitors

Inhibitors Comment Organism Structure
CO reversed by illumination Oryctolagus cuniculus
hydroxylamine sulfate 0.1 mM causes 79% inhibition, 1 mM causes 94% inhibition Oryctolagus cuniculus
KCN
-
 Oryctolagus cuniculus
L-tryptophan substrate inhibition Oryctolagus cuniculus
additional information not inhibited by catalase and D-tryptophan Oryctolagus cuniculus
Sodium azide 1 mM causes 20% inhibition Oryctolagus cuniculus
Tiron 1 mM causes 40% inhibition Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-tryptophan + O2 Oryctolagus cuniculus
-
 N-formyl-D-kynurenine
-
 ?
L-tryptophan + O2 Oryctolagus cuniculus
-
 N-formyl-L-kynurenine
-
 ?

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
homogenization, ammonium sulfate precipitation Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
intestine
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.01
-
 ascending colon, 0.05 mM potassium phosphate buffer (pH 7.5), 0.005 mM methylene blue, 0.01 mM ascorbic acid, 0.003 mM tryptophan, 37°C Oryctolagus cuniculus
0.01
-
 caecum, 0.05 mM potassium phosphate buffer (pH 7.5), 0.005 mM methylene blue, 0.01 mM ascorbic acid, 0.003 mM tryptophan, 37°C Oryctolagus cuniculus
0.04
-
 small intestine IV, 0.05 mM potassium phosphate buffer (pH 7.5), 0.005 mM methylene blue, 0.01 mM ascorbic acid, 0.003 mM tryptophan, 37°C Oryctolagus cuniculus
0.05
-
 small intestine proximal portion, 0.05 mM potassium phosphate buffer (pH 7.5), 0.005 mM methylene blue, 0.01 mM ascorbic acid, 0.003 mM tryptophan, 37°C Oryctolagus cuniculus
0.07
-
 small intestine V, 0.05 mM potassium phosphate buffer (pH 7.5), 0.005 mM methylene blue, 0.01 mM ascorbic acid, 0.003 mM tryptophan, 37°C Oryctolagus cuniculus
0.08
-
 appendix, 0.05 mM potassium phosphate buffer (pH 7.5), 0.005 mM methylene blue, 0.01 mM ascorbic acid, 0.003 mM tryptophan, 37°C Oryctolagus cuniculus
0.15
-
 appendix, 0.05 mM potassium phosphate buffer (pH 7.5), 0.005 mM methylene blue, 0.01 mM ascorbic acid, 0.003 mM tryptophan, 37°C Oryctolagus cuniculus
5.19
-
 small intestine VI, 0.05 mM potassium phosphate buffer (pH 7.5), 0.005 mM methylene blue, 0.01 mM ascorbic acid, 0.003 mM tryptophan, 37°C Oryctolagus cuniculus
6.97
-
 small intestine distal portion, 0.05 mM potassium phosphate buffer (pH 7.5), 0.005 mM methylene blue, 0.01 mM ascorbic acid, 0.003 mM tryptophan, 37°C Oryctolagus cuniculus
234
-
 enzyme after purification, 0.05 mM potassium phosphate buffer (pH 7.5), 0.005 mM methylene blue, 0.01 mM ascorbic acid, 0.003 mM tryptophan, 37°C Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-tryptophan + O2
-
 Oryctolagus cuniculus N-formyl-D-kynurenine
-
 ?
L-tryptophan + O2
-
 Oryctolagus cuniculus N-formyl-L-kynurenine
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 L-tryptophan Oryctolagus cuniculus
7.5
-
 D-tryptophan Oryctolagus cuniculus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8 5% activity loss at pH 5.5, 10% activity loss at pH 7, 20% activity loss at pH 8 Oryctolagus cuniculus

Cofactor

Cofactor Comment Organism Structure
methylene blue stimulates the formation of D-kynurenine Oryctolagus cuniculus
additional information ascorbic acid, can be replaced by xanthine oxidase with hypoxanthine Oryctolagus cuniculus
additional information not affected by hydrogen peroxide Oryctolagus cuniculus
additional information toluidine blue, can replace methylene blue Oryctolagus cuniculus