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Literature summary for 1.13.11.52 extracted from

  • Austin, C.J.; Mailu, B.M.; Maghzal, G.J.; Sanchez-Perez, A.; Rahlfs, S.; Zocher, K.; Yuasa, H.J.; Arthur, J.W.; Becker, K.; Stocker, R.; Hunt, N.H.; Ball, H.J.
    Biochemical characteristics and inhibitor selectivity of mouse indoleamine 2,3-dioxygenase-2 (2010), Amino Acids, 39, 565-578.
    View publication on PubMed
Search for more literature for 1.13.11.52

Activating Compound

Activating Compound Comment Organism Structure
cytochrome b5
-
 Mus musculus
methylene blue
-
 Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
isozyme IDO2 is expressed in KRX cells, isozyme IDO1 is expressed in Escherichia coli Rosetta cells Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
1-methyl-D-tryptophan poor non-competitive inhibitor of both IDO1 and IDO2 activity Mus musculus
1-methyl-L-tryptophan poor competitive inhibitor of isozyme IDO1 Mus musculus
nitric oxide nitric oxide can potentially inhibit isozyme IDO2 activity Mus musculus
norharman
-
 Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.028
-
 L-tryptophan recombinant isozyme IDO1, in the presence of 0.01 mM methylene blue, in 100 mM phosphate buffer, pH 7.4, at 37°C Mus musculus
0.029
-
 L-tryptophan recombinant isozyme IDO1, in the presence of 50 nM recombinant human cytochrome b5 and 50 nM NADPH cytochrome P450 reductase, in 100 mM phosphate buffer, pH 7.4, at 37°C Mus musculus
0.53
-
 L-tryptophan recombinant isozyme IDO2, in the presence of 50 nM recombinant human cytochrome b5 and 50 nM NADPH cytochrome P450 reductase, in 100 mM phosphate buffer, pH 7.4, at 37°C Mus musculus
12
-
 L-tryptophan recombinant isozyme IDO2, in the presence of 0.01 mM methylene blue, in 100 mM phosphate buffer, pH 7.4, at 37°C Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 isozymes IDO1 and IDO2, SDS-PAGE Mus musculus
45200
-
 isozyme IDO2, calculated from amino acid sequence Mus musculus
45300
-
 isozyme IDO1, calculated from amino acid sequence Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-tryptophan + O2 Mus musculus
-
 N-formyl-D-kynurenine
-
 ?
L-tryptophan + O2 Mus musculus
-
 N-formyl-L-kynurenine
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-hydroxy-L-tryptophan + O2
-
 Mus musculus N-formyl-5-hydroxy-L-kynurenine
-
 ?
D-tryptophan + O2
-
 Mus musculus N-formyl-D-kynurenine
-
 ?
L-tryptophan + O2
-
 Mus musculus N-formyl-L-kynurenine
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 45000, isozymes IDO1 and IDO2, SDS-PAGE Mus musculus

Synonyms

Synonyms Comment Organism
IDO1 isozyme Mus musculus
IDO2 isozyme Mus musculus
indoleamine 2,3-dioxygenase-1
-
 Mus musculus
indoleamine 2,3-dioxygenase-2
-
 Mus musculus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
48 60 the melting temperature of the denaturation process for recombinant isozyme IDO1 is 60°C, with a possible second intermediate at 48°C, recombinant isozyme IDO2 denatures in a single step, with a melting temperature at 48°C Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5 isozyme IDO1 Mus musculus
7.4 7.5 isozyme IDO2 Mus musculus

Cofactor

Cofactor Comment Organism Structure
heme recombinant isozymes IDO2 and IDO1 are ferric (Fe3+) type heme proteins Mus musculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.08
-
 1-methyl-L-tryptophan recombinant isozyme IDO1, in the presence of 50 nM recombinant human cytochrome b5 and 50 nM NADPH cytochrome P450 reductase, in 100 mM phosphate buffer, pH 7.4, at 37°C Mus musculus
0.105
-
 1-methyl-L-tryptophan recombinant isozyme IDO1, in the presence of 0.01 mM methylene blue, in 100 mM phosphate buffer, pH 7.4, at 37°C Mus musculus
0.44
-
 1-methyl-L-tryptophan recombinant isozyme IDO2, in the presence of 50 nM recombinant human cytochrome b5 and 50 nM NADPH cytochrome P450 reductase, in 100 mM phosphate buffer, pH 7.4, at 37°C Mus musculus
0.69
-
 norharman recombinant isozyme IDO1, in the presence of 0.01 mM methylene blue, in 100 mM phosphate buffer, pH 7.4, at 37°C Mus musculus
1.08
-
 norharman recombinant isozyme IDO1, in the presence of 0.01 mM methylene blue, in 100 mM phosphate buffer, pH 7.4, at 37°C Mus musculus
1.73
-
 norharman recombinant isozyme IDO1, in the presence of 50 nM recombinant human cytochrome b5 and 50 nM NADPH cytochrome P450 reductase, in 100 mM phosphate buffer, pH 7.4, at 37°C Mus musculus
1.96
-
 norharman recombinant isozyme IDO2, in the presence of 50 nM recombinant human cytochrome b5 and 50 nM NADPH cytochrome P450 reductase, in 100 mM phosphate buffer, pH 7.4, at 37°C Mus musculus
2.75
-
 1-methyl-L-tryptophan recombinant isozyme IDO1, in the presence of 0.01 mM methylene blue, in 100 mM phosphate buffer, pH 7.4, at 37°C Mus musculus
11.3
-
 1-methyl-D-tryptophan recombinant isozyme IDO1, in the presence of 0.01 mM methylene blue, in 100 mM phosphate buffer, pH 7.4, at 37°C Mus musculus