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Literature summary for 1.13.11.52 extracted from

  • Kuo, H.H.; Mauk, A.G.
    Indole peroxygenase activity of indoleamine 2,3-dioxygenase (2012), Proc. Natl. Acad. Sci. USA, 109, 13966-13971.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.52

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression in Escherichia coli Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics of IDO-catalyzed indole oxidation as supported by H2O2, overview. The rate of disappearance of indole as a function of [H2O2] in the IDOFe3+-catalyzed reaction exhibits Michaelis-Menten behavior, with Km of about 1.1 mM for peroxide [indole] Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ heme-containing enzyme Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-tryptophan + O2 Homo sapiens IDO1 N-formyl-D-kynurenine
-
 ?
L-tryptophan + O2 Homo sapiens IDO1 and IDO2 N-formyl-L-kynurenine
-
 ?
additional information Homo sapiens the enzyme catalyzes the oxidation of indole by H2O2, with generation of 2- and 3-oxoindole as the major products, in the absence of O2 and reducing agents and is not inhibited by superoxide dismutase or hydroxyl radical scavengers, although it is strongly inhibited by L-Trp. IDO inserts oxygen into indole in a reaction that is mechanistically analogous to the peroxide shunt pathway of cytochrome P450 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli involving affinity chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-tryptophan + O2 IDO1 Homo sapiens N-formyl-D-kynurenine
-
 ?
L-tryptophan + O2 IDO1 and IDO2 Homo sapiens N-formyl-L-kynurenine
-
 ?
additional information the enzyme catalyzes the oxidation of indole by H2O2, with generation of 2- and 3-oxoindole as the major products, in the absence of O2 and reducing agents and is not inhibited by superoxide dismutase or hydroxyl radical scavengers, although it is strongly inhibited by L-Trp. IDO inserts oxygen into indole in a reaction that is mechanistically analogous to the peroxide shunt pathway of cytochrome P450 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
IDO
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
heme
-
 Homo sapiens