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Literature summary for 1.13.11.54 extracted from

  • Friedman, E.; Wang, H.; Jiang, K.; Perovic, I.; Deshpande, A.; Pochapsky, T.; Temple, B.; Hicks, S.; Harden, T.; Jones, A.
    Acireductone dioxygenase 1 (ARD1) is an effector of the heterotrimeric G protein beta subunit in Arabidopsis (2011), J. Biol. Chem., 286, 30107-30118.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.54

Activating Compound

Activating Compound Comment Organism Structure
Arabidopsis thaliana G protein beta possible mechanism for Arabidopsis thaliana G protein beta activation of acireductone dioxygenase 1 activity, overview Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
transient expression of GFP-acireductone dioxygenase 1 in Arabidopsis thaliana protoplasts. The coding regions of ARD1–4 are cloned into pDEST17TM and pDEST15TM containing an N-terminal poly-His tag and an N-terminal GST tag, respectively, and are expressed in Escherichia coli strain Rosetta BL21 Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information usage of three tDNA insertion mutant alleles, ard1-1 (SALK_119327), ard1-2 (GABI_595C04), and ard1-3 (SALK_034308). Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ dependent on, acireductone dioxygenase 1 is an active metalloenzyme, Fe2+ is active site bound Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 Arabidopsis thaliana
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 4-(methylthio)-2-oxobutanoate + formate
-
 ?
1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 Arabidopsis thaliana Columbia-0
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 4-(methylthio)-2-oxobutanoate + formate
-
 ?
additional information Arabidopsis thaliana acireductone dioxygenase 1 primarily performs the on-pathway reaction with bound Fe2+ as metal cofactor. Acireductone dioxygenase 1 physically interacts with its regulator Arabidopsis thaliana G protein beta, identification of a potential Arabidopsis thaliana G protein beta-acireductone dioxygenase 1 interface, overview ?
-
 ?
additional information Arabidopsis thaliana Columbia-0 acireductone dioxygenase 1 primarily performs the on-pathway reaction with bound Fe2+ as metal cofactor. Acireductone dioxygenase 1 physically interacts with its regulator Arabidopsis thaliana G protein beta, identification of a potential Arabidopsis thaliana G protein beta-acireductone dioxygenase 1 interface, overview ?
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 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q0WSS4
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-
Arabidopsis thaliana Columbia-0 Q0WSS4
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-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged acireductone dioxygenase 1 and GST-tagged acireductone dioxygenase 1 by nickel affinity and glutathione affinity chromatographies, respectively Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2
-
 Arabidopsis thaliana 4-(methylthio)-2-oxobutanoate + formate
-
 ?
1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2
-
 Arabidopsis thaliana Columbia-0 4-(methylthio)-2-oxobutanoate + formate
-
 ?
additional information acireductone dioxygenase 1 primarily performs the on-pathway reaction with bound Fe2+ as metal cofactor. Acireductone dioxygenase 1 physically interacts with its regulator Arabidopsis thaliana G protein beta, identification of a potential Arabidopsis thaliana G protein beta-acireductone dioxygenase 1 interface, overview Arabidopsis thaliana ?
-
 ?
additional information acireductone dioxygenase 1 primarily performs the on-pathway reaction with bound Fe2+ as metal cofactor. Acireductone dioxygenase 1 physically interacts with its regulator Arabidopsis thaliana G protein beta, identification of a potential Arabidopsis thaliana G protein beta-acireductone dioxygenase 1 interface, overview Arabidopsis thaliana Columbia-0 ?
-
 ?

Subunits

Subunits Comment Organism
More homology modeling of acireductone dioxygenase 1, overview Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
acireductone dioxygenase 1
-
 Arabidopsis thaliana
ARD1
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 Arabidopsis thaliana

General Information

General Information Comment Organism
additional information identification of potential effectors in the Arabidopsis thaliana G protein beta signaling pathway by an activation tagging approach to randomly create dominant suppressors of agb1-2, overview. Homology modeling of acireductone dioxygenase 1, overview Arabidopsis thaliana
physiological function one function of acireductone dioxygenases is to operate in the methionine salvage pathway. Acireductone dioxygenase 1 physically interacts with Arabidopsis thaliana G protein beta, and acireductone dioxygenase enzymatic activity is stimulated by Arabidopsis thaliana G protein beta in vitro. Overexpression of acireductone dioxygenase 1 suppresses the 2-day-old etiolated phenotype of agb1-2. Acireductone dioxygenase 1 and Arabidopsis thaliana G protein beta both control embryonic hypocotyl length by modulating cell division. They also may contribute to the production of ethylene, a product of the methionine salvage pathway. Arabidopsis thaliana G protein beta regulates acireductone dioxygenase 1 activity to control cell division, possible mechanism for Arabidopsis thaliana G protein beta activation of acireductone dioxygenase 1 activity, overview Arabidopsis thaliana