Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 100 mM, slight stimulatory effect | Acidianus ambivalens |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli results in active, soluble SOR and in inclusion bodies from which active SOR can be refolded as long as ferric ions are present in the refolding solution. Wild-type, recombinant and refolded enzyme possesses indistinguishable properties | Acidianus ambivalens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | 0.5 mM, 95% inhibition of oxygenase reaction (formation of hydrogen sulfide), 84% inhibition of reductase reaction (formation of sulfite plus thiosulfate) | Acidianus ambivalens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | 3 | sulfur | pH 7.2, 65°C, oxygenase reaction (formation of hydrogen sulfide) | Acidianus ambivalens | |
13 | - |
sulfur | pH 7.2, 65°C, reductase reaction (formation of sulphite plus thiosulfate) | Acidianus ambivalens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | the enzyme contains a low-potential mononuclear non-heme iron centre, which has a reduction potential of -268 mV at pH 6.5, one iron atom per subunit | Acidianus ambivalens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
24 * 36000, ball-shaped assembly with a central hollow core probably consisting of 24 subunits in a 432 symmetry, the subunits form homodimers as the building blocks of the holoenzyme, SDS-PAGE | Acidianus ambivalens |
732000 | - |
non-denaturing PAGE | Acidianus ambivalens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4 sulfur + 4 H2O + O2 | Acidianus ambivalens | initial enzyme in the sulfur-oxidation pathway | 2 hydrogen sulfide + 2 bisulfite + 2 H+ | - |
? | |
4 sulfur + 4 H2O + O2 | Acidianus ambivalens DSM 3772 | initial enzyme in the sulfur-oxidation pathway | 2 hydrogen sulfide + 2 bisulfite + 2 H+ | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
guanidine-HCl | reversible denaturation curve with midpoint at 3.4 M guanidine-HCl | Acidianus ambivalens |
urea | reversible denaturation curve with midpoints at 5.9 M urea | Acidianus ambivalens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidianus ambivalens | P29082 | - |
- |
Acidianus ambivalens DSM 3772 | P29082 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Acidianus ambivalens |
Renatured (Comment) | Organism |
---|---|
reversible denaturation curves with midpoints at 3.4 M guanidine-HCl and 5.9 M urea | Acidianus ambivalens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4 sulfur + 4 H2O + O2 | - |
Acidianus ambivalens | 2 hydrogen sulfide + 2 bisulfite + 2 H+ | - |
? | |
4 sulfur + 4 H2O + O2 | initial enzyme in the sulfur-oxidation pathway | Acidianus ambivalens | 2 hydrogen sulfide + 2 bisulfite + 2 H+ | - |
? | |
4 sulfur + 4 H2O + O2 | - |
Acidianus ambivalens DSM 3772 | 2 hydrogen sulfide + 2 bisulfite + 2 H+ | - |
? | |
4 sulfur + 4 H2O + O2 | initial enzyme in the sulfur-oxidation pathway | Acidianus ambivalens DSM 3772 | 2 hydrogen sulfide + 2 bisulfite + 2 H+ | - |
? | |
additional information | not active with tetrathionate | Acidianus ambivalens | ? | - |
? | |
additional information | not active with tetrathionate | Acidianus ambivalens DSM 3772 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetraicosamer | 24 * 36000, ball-shaped assembly with a central hollow core probably consisting of 24 subunits in a 432 symmetry, the subunits form homodimers as the building blocks of the holoenzyme, SDS-PAGE | Acidianus ambivalens |
Synonyms | Comment | Organism |
---|---|---|
SOR | - |
Acidianus ambivalens |
sulphur oxygenase reductase | - |
Acidianus ambivalens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
sulfur | pH 7.2, 65°C, reductase reaction (formation of sulfite plus thiosulfate) | Acidianus ambivalens | |
2.2 | - |
sulfur | pH 7.2, 65°C, oxygenase reaction (formation of hydrogen sulfide) | Acidianus ambivalens |
General Information | Comment | Organism |
---|---|---|
physiological function | initial enzyme in the sulfur-oxidation pathway | Acidianus ambivalens |