Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.13.11.57 extracted from

  • Kasai, D.; Masai, E.; Miyauchi, K.; Katayama, Y.; Fukuda, M.
    Characterization of the gallate dioxygenase gene: three distinct ring cleavage dioxygenases are involved in syringate degradation by Sphingomonas paucimobilis SYK-6 (2005), J. Bacteriol., 187, 5067-5074.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.57

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Sphingomonas paucimobilis

Inhibitors

Inhibitors Comment Organism Structure
EDTA complete inhibition at 0.5 mM, the oxygen consumption activity after EDTA treatment is completely recovered by addition of Fe2+ (119%) Sphingomonas paucimobilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0669
-
 gallate in 50 mM GTA buffer (pH 8.5) consisting of 50 mM 3,3-dimethylglutarate, 50 mM Tris, and 50 mM 2-amino-2-methyl-1,3-propanediol, at 30°C Sphingomonas paucimobilis

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ DesB requires Fe2+ for activity Sphingomonas paucimobilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 2 * 45000, SDS-PAGE Sphingomonas paucimobilis
46843
-
 2 * 46843, calculated from amino acid sequence Sphingomonas paucimobilis
86000
-
 gel filtration Sphingomonas paucimobilis

Organism

Organism UniProt Comment Textmining
Sphingomonas paucimobilis Q5NTE5
-
-
Sphingomonas paucimobilis SYK-6 Q5NTE5
-
-

Purification (Commentary)

Purification (Comment) Organism
POROS polyethyleneimine column chromatography Sphingomonas paucimobilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
gallate + O2
-
 Sphingomonas paucimobilis 4-oxalomesaconate
-
 ?
gallate + O2
-
 Sphingomonas paucimobilis SYK-6 4-oxalomesaconate
-
 ?
additional information no activity with protocatechuate, 3-O-methylgallate, gentisate, 2,3-dihydroxybiphenyl, 4-methylcatechol, 2,3-dihydroxybenzoate, and methylgallate Sphingomonas paucimobilis ?
-
 ?
additional information no activity with protocatechuate, 3-O-methylgallate, gentisate, 2,3-dihydroxybiphenyl, 4-methylcatechol, 2,3-dihydroxybenzoate, and methylgallate Sphingomonas paucimobilis SYK-6 ?
-
 ?
pyrogallol + O2
-
 Sphingomonas paucimobilis ?
-
 ?
pyrogallol + O2
-
 Sphingomonas paucimobilis SYK-6 ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 45000, SDS-PAGE Sphingomonas paucimobilis
homodimer 2 * 46843, calculated from amino acid sequence Sphingomonas paucimobilis

Synonyms

Synonyms Comment Organism
DesB
-
 Sphingomonas paucimobilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Sphingomonas paucimobilis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 40
-
 Sphingomonas paucimobilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Sphingomonas paucimobilis

pH Range

pH Minimum pH Maximum Comment Organism
6 9
-
 Sphingomonas paucimobilis

General Information

General Information Comment Organism
physiological function the enzyme is involved in syringate catabolism, the pathway involving DesB plays an especially important role in the growth of Sphingomonas paucimobilis SYK-6 on syringate, and DesB is involved in gallate degradation Sphingomonas paucimobilis