Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas fluorescens |
Protein Variants | Comment | Organism |
---|---|---|
H52A | inactive enzyme | Pseudomonas fluorescens |
H93A | 24.8% activity of the native enzyme | Pseudomonas fluorescens |
H96A | inactive enzyme | Pseudomonas fluorescens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | Fe2+ chelator, 1 mM, complete inhibition | Pseudomonas fluorescens | |
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide methiodide | carboxyl-directed reagent, 1 mM, 26% inhibition | Pseudomonas fluorescens | |
2,2'-dipyridyl | Fe2+ chelator, 1 mM, complete inhibition | Pseudomonas fluorescens | |
Cd2+ | 0.1 mM, more than 99% inhibition | Pseudomonas fluorescens | |
Co2+ | 1 mM, 92% inhibition | Pseudomonas fluorescens | |
Cu2+ | 0.1 mM, complete inhibition | Pseudomonas fluorescens | |
diethyl dicarbonate | modifies histidine residues of catechol dioxygenases, 1 mM, 70% inhibition | Pseudomonas fluorescens | |
Dithionitrobenzoic acid | cysteine-directed reagent, 1 mM, complete inhibition | Pseudomonas fluorescens | |
EDTA | 1 mM, 99% inhibition | Pseudomonas fluorescens | |
Fe3+ | 0.1 mM, 82% inhibition | Pseudomonas fluorescens | |
iodoacetate | cysteine-directed reagent, 1 mM, 42% inhibition | Pseudomonas fluorescens | |
Ni2+ | 1 mM, 82% inhibition | Pseudomonas fluorescens | |
Zn2+ | 0.1 mM, 99% inhibition | Pseudomonas fluorescens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | needed for catalytic activity | Pseudomonas fluorescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21240 | - |
predicted from cDNA sequence | Pseudomonas fluorescens |
23800 | - |
SDS-PAGE | Pseudomonas fluorescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxyanthranilate + O2 | Pseudomonas fluorescens | - |
2-amino-3-carboxymuconate semialdehyde | - |
? | |
3-hydroxyanthranilate + O2 | Pseudomonas fluorescens KU-7 | - |
2-amino-3-carboxymuconate semialdehyde | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | Q83V26 | - |
- |
Pseudomonas fluorescens KU-7 | Q83V26 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.9 | - |
3-hydroxyanthranilate as substrate, pH 6.5, 30ºC | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxyanthranilate + O2 | - |
Pseudomonas fluorescens | 2-amino-3-carboxymuconate semialdehyde | - |
? | |
3-hydroxyanthranilate + O2 | - |
Pseudomonas fluorescens KU-7 | 2-amino-3-carboxymuconate semialdehyde | - |
? | |
additional information | no detectable activity with 3-amino-4-hydroxybenzoic acid, 4-aminoresorcinol, 2-amino-m-cresol, 4-amino-3-hydroxybenzoic acid, 3-aminosalicylic acid, 6-amino-m-cresol, 3-methylcatechol, 4-methylcatechol, 1,2,4-trihydroxybenzene, 2,3-dihydroxybenzoic acid, 4-amino-m-cresol, 5-aminosalicylic acid, gentisic acid, homogentisic acid, 2-amino-4-chlorophenol, 2-amino-p-cresol, catechol, 1,2,3-trihydroxybenzene, protocatechuic acid, hydroquinone as substrates | Pseudomonas fluorescens | ? | - |
? | |
additional information | no detectable activity with 3-amino-4-hydroxybenzoic acid, 4-aminoresorcinol, 2-amino-m-cresol, 4-amino-3-hydroxybenzoic acid, 3-aminosalicylic acid, 6-amino-m-cresol, 3-methylcatechol, 4-methylcatechol, 1,2,4-trihydroxybenzene, 2,3-dihydroxybenzoic acid, 4-amino-m-cresol, 5-aminosalicylic acid, gentisic acid, homogentisic acid, 2-amino-4-chlorophenol, 2-amino-p-cresol, catechol, 1,2,3-trihydroxybenzene, protocatechuic acid, hydroquinone as substrates | Pseudomonas fluorescens KU-7 | ? | - |
? |