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Literature summary for 1.13.11.6 extracted from

  • Li, X.; Guo, M.; Fan, J.; Tang, W.; Wang, D.; Ge, H.; Rong, H.; Teng, M.; Niu, L.; Liu, Q.; Hao, Q.
    Crystal structure of 3-hydroxyanthranilic acid 3,4-dioxygenase from Saccharomyces cerevisiae: a special subgroup of the type III extradiol dioxygenases (2006), Protein Sci., 15, 761-773.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
Y3HAO protein is subcloned into the pET-28a expression vector from extracted Saccharomyces cerevisiae genomic DNA, and the Y3HAO protein is highly expressed as a soluble protein in Escherichia coli strain BL21(DE3) with a six-residueHis tag attached to its N-terminus Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop-vapor diffusion method, 2.4 A resolution Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ non-heme ferrous enzyme Saccharomyces cerevisiae
Ni2+ two nickel binding sites per molecule. One of the bound nickel atoms occupies the proposed ferrous-coordinated active site Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-hydroxyanthranilate + O2 Saccharomyces cerevisiae in the kynurenine pathway 2-amino-3-carboxymuconate semialdehyde
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 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
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-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxyanthranilate + O2 in the kynurenine pathway Saccharomyces cerevisiae 2-amino-3-carboxymuconate semialdehyde
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 ?

Cofactor

Cofactor Comment Organism Structure
additional information non-heme enzyme Saccharomyces cerevisiae