Cloned (Comment) | Organism |
---|---|
expressed in Escherichia ER2566 cells | Penicillium oxalicum |
Protein Variants | Comment | Organism |
---|---|---|
C1005S | the variant exhibits only 8-HPODE formation without 6,8-DiHODE accumulation | Penicillium oxalicum |
D749A | the mutant shows less than 10% activity of the wild type enzyme | Penicillium oxalicum |
E728A | the mutant shows less than 10% activity of the wild type enzyme | Penicillium oxalicum |
F794E | the mutant acts as 7,8-linoleate diol synthase | Penicillium oxalicum |
F794Y | the mutant shows less than 10% activity of the wild type enzyme | Penicillium oxalicum |
F798Y | the mutant shows less than 10% activity of the wild type enzyme | Penicillium oxalicum |
H1003A | the mutant shows less than 10% activity of the wild type enzyme | Penicillium oxalicum |
I841A | the mutant shows more than 110% activity of the wild type enzyme | Penicillium oxalicum |
L970A | the mutant shows more than 110% activity of the wild type enzyme | Penicillium oxalicum |
M1051A | the mutant shows more than 110% activity of the wild type enzyme | Penicillium oxalicum |
M695A | the mutant shows more than 110% activity of the wild type enzyme | Penicillium oxalicum |
M883A | the mutant shows more than 110% activity of the wild type enzyme | Penicillium oxalicum |
N886D | the variant exhibits only 8-HPODE formation without 6,8-DiHODE accumulation | Penicillium oxalicum |
P1016A | the mutant shows more than 110% activity of the wild type enzyme | Penicillium oxalicum |
Q887A | the mutant shows less than 10% activity of the wild type enzyme | Penicillium oxalicum |
Q889A | the mutant acts as 8,11-linoleate diol synthase | Penicillium oxalicum |
Q889A | the variant exhibits only 8-HPODE formation without 6,8-DiHODE accumulation | Penicillium oxalicum |
R707A | the variant exhibits only 8-HPODE formation without 6,8-DiHODE accumulation | Penicillium oxalicum |
R814A | the mutant shows less than 10% activity of the wild type enzyme | Penicillium oxalicum |
R934A | the variant exhibits only 8-HPODE formation without 6,8-DiHODE accumulation | Penicillium oxalicum |
T879A | the mutant shows more than 110% activity of the wild type enzyme | Penicillium oxalicum |
V1010A | the mutant shows more than 110% activity of the wild type enzyme | Penicillium oxalicum |
V884A | the mutant shows more than 110% activity of the wild type enzyme | Penicillium oxalicum |
Y690F | the mutant shows less than 10% activity of the wild type enzyme | Penicillium oxalicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0264 | - |
linoleic acid | full-length enzyme, at pH 6.5 and 25°C | Penicillium oxalicum | |
0.0283 | - |
oleic acid | full-length enzyme, at pH 6.5 and 25°C | Penicillium oxalicum | |
0.0329 | - |
alpha-linolenic acid | full-length enzyme, at pH 6.5 and 25°C | Penicillium oxalicum | |
0.0475 | - |
palmitoleic acid | full-length enzyme, at pH 6.5 and 25°C | Penicillium oxalicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
370000 | - |
gel filtration | Penicillium oxalicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
linoleate + O2 | Penicillium oxalicum | - |
(8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoate | - |
? | |
linoleate + O2 | Penicillium oxalicum KCTC 6440 | - |
(8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium oxalicum | - |
- |
- |
Penicillium oxalicum KCTC 6440 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His-Trap column chromatography | Penicillium oxalicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-linolenic acid + O2 | - |
Penicillium oxalicum | (6S,8R,9Z,12Z,15Z)-dihydroxy-9,12,15-octadecatrienoic acid | - |
? | |
alpha-linolenic acid + O2 | - |
Penicillium oxalicum KCTC 6440 | (6S,8R,9Z,12Z,15Z)-dihydroxy-9,12,15-octadecatrienoic acid | - |
? | |
linoleate + O2 | - |
Penicillium oxalicum | (8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoate | - |
? | |
linoleate + O2 | - |
Penicillium oxalicum KCTC 6440 | (8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoate | - |
? | |
linoleic acid + O2 | highest activity | Penicillium oxalicum | (6R,8R)-dihydroxy-9,12(Z,Z)-octadecadienoic acid | - |
? | |
linoleic acid + O2 | highest activity | Penicillium oxalicum KCTC 6440 | (6R,8R)-dihydroxy-9,12(Z,Z)-octadecadienoic acid | - |
? | |
oleic acid + O2 | - |
Penicillium oxalicum | (6R,8R,9Z)-dihydroxy-9-octadecenoic acid | - |
? | |
oleic acid + O2 | - |
Penicillium oxalicum KCTC 6440 | (6R,8R,9Z)-dihydroxy-9-octadecenoic acid | - |
? | |
palmitoleic acid + O2 | - |
Penicillium oxalicum | (6S,8R,9Z)-dihydroxy-9-hexadecenoic acid | - |
? | |
palmitoleic acid + O2 | - |
Penicillium oxalicum KCTC 6440 | (6S,8R,9Z)-dihydroxy-9-hexadecenoic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotrimer | 3 * 121000, full-length enzyme, SDS-PAGE | Penicillium oxalicum |
Synonyms | Comment | Organism |
---|---|---|
6,8-diol synthase | - |
Penicillium oxalicum |
6R,8R-linoleate diol synthase | - |
Penicillium oxalicum |
6R,8RLDS | - |
Penicillium oxalicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Penicillium oxalicum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
the enzyme exhibits 28% of its initial activity at 50°C after 2 h | Penicillium oxalicum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.34 | - |
alpha-linolenic acid | full-length enzyme, at pH 6.5 and 25°C | Penicillium oxalicum | |
21.4 | - |
palmitoleic acid | full-length enzyme, at pH 6.5 and 25°C | Penicillium oxalicum | |
30.4 | - |
oleic acid | full-length enzyme, at pH 6.5 and 25°C | Penicillium oxalicum | |
32.8 | - |
linoleic acid | full-length enzyme, at pH 6.5 and 25°C | Penicillium oxalicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Penicillium oxalicum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
130 | - |
alpha-linolenic acid | full-length enzyme, at pH 6.5 and 25°C | Penicillium oxalicum | |
450 | - |
palmitoleic acid | full-length enzyme, at pH 6.5 and 25°C | Penicillium oxalicum | |
1070 | - |
oleic acid | full-length enzyme, at pH 6.5 and 25°C | Penicillium oxalicum | |
1240 | - |
linoleic acid | full-length enzyme, at pH 6.5 and 25°C | Penicillium oxalicum |