Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.13.11.63 extracted from

  • Oberhauser, V.; Voolstra, O.; Bangert, A.; von Lintig, J.; Vogt, K.
    NinaB combines carotenoid oxygenase and retinoid isomerase activity in a single polypeptide (2008), Proc. Natl. Acad. Sci. USA, 105, 19000-19005.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.63

Cloned(Commentary)

Cloned (Comment) Organism
for expression in Escherichia coli cells, genetically engineered to produce beta-carotene Mus musculus
for expression in Escherichia coli cells, genetically engineered to produce beta-carotene Drosophila melanogaster
into the vectors pCRII TOPO and pBAD-TOPO for expression in Escherichia coli XL1-blue cells Galleria mellonella

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0104
-
 beta-carotene
-
 Galleria mellonella
0.02213
-
 alpha-carotene
-
 Galleria mellonella

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57800
-
 theoretical Galleria mellonella
64000
-
 determined by SDS-PAGE and Western Blot analysis Galleria mellonella

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster Q9VFS2
-
-
Galleria mellonella
-
-
-
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by affinity chromatography Galleria mellonella

Source Tissue

Source Tissue Comment Organism Textmining
head
-
 Galleria mellonella
-
pupa
-
 Galleria mellonella
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-carotene + ?
-
 Galleria mellonella 11-cis-retinal + all-trans-retinal + all-trans-alpha-retinal 32.7% 11-cis-retinal + 17.3% all-trans-retinal + 50.0% all-trans-alpha-retinal ?
beta-carotene + ?
-
 Galleria mellonella 11-cis-retinal + all-trans-retinal 41.2% 11-cis-retinal + 58.8% all-trans-retinal ?
beta-carotene + O2
-
 Drosophila melanogaster 11-cis-retinal + 13-cis-retinal + all-trans-retinal
-
 ?
beta-carotene + O2
-
 Mus musculus 13-cis-retinal + all-trans-retinal
-
 ?
cryptoxanthin + O2
-
 Galleria mellonella 11-cis-retinal + all-trans-retinal + 11-cis-3-hydroxy-retinal + all-trans-3-hydroxy-retinal 4.2% 11-cis-retinal + 45.8% all-trans-retinal + 35.1% 11-cis-3-hydroxy-retinal + 14.9% all-trans-3-hydroxy-retinal ?
lutein + O2
-
 Galleria mellonella 11-cis-3-hydroxy-retinal + all-trans-3-hydroxy-retinal + all-trans-3-hydroxy-alpha-retinal 39.9% 11-cis-3-hydroxy-retinal + 10.1% all-trans-3-hydroxy-retinal + 47.7% all-trans-3-hydroxy-alpha-retinal ?
zeaxanthin + O2
-
 Galleria mellonella (3R)-11-cis-3-hydroxyretinal + (3R)-all-trans-3-hydroxyretinal 41.5% 11-cis-3-hydroxy-retinal + 58.5% all-trans-3-hydroxy-retinal ?

Synonyms

Synonyms Comment Organism
carotenoid oxygenase
-
 Mus musculus
carotenoid oxygenase
-
 Galleria mellonella
carotenoid oxygenase
-
 Drosophila melanogaster
CMO1
-
 Mus musculus
NinaB
-
 Mus musculus
NinaB
-
 Galleria mellonella
NinaB
-
 Drosophila melanogaster

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
 activity assay Galleria mellonella

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00817
-
 alpha-carotene
-
 Galleria mellonella
0.00867
-
 beta-carotene
-
 Galleria mellonella

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 activity assay Galleria mellonella