General Stability | Organism |
---|---|
dialysis against 50 mM MOPS (pH 7.3) containing ethanol (10% [v/v]) for 24 h abolishes 95% of the activity of 2-aminophenol 1,6-dioxygenase | Pseudomonas oleovorans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,2,3-trihydroxybenzene | complete inhibition at 0.05 mM | Pseudomonas oleovorans | |
1,2,4-Trihydroxybenzene | complete inhibition at 0.05 mM | Pseudomonas oleovorans | |
2,2'-dipyridyl | complete inhibition at 2 mM | Pseudomonas oleovorans | |
2,3-Dihydroxybenzoic acid | 20% inhibition at 0.05 mM | Pseudomonas oleovorans | |
2-amino-p-cresol | 90% inhibition at 0.05 mM | Pseudomonas oleovorans | |
2-methoxyphenol | 30% inhibition at 0.05 mM | Pseudomonas oleovorans | |
3-Amino-4-hydroxybenzoic acid | 20% inhibition at 0.05 mM | Pseudomonas oleovorans | |
3-chlorocatechol | complete inhibition at 0.05 mM | Pseudomonas oleovorans | |
3-hydroxyanthranilic acid | 20% inhibition at 0.05 mM | Pseudomonas oleovorans | |
3-methylcatechol | complete inhibition at 0.05 mM | Pseudomonas oleovorans | |
4-amino-3-hydroxybenzoic acid | 30% inhibition at 0.05 mM | Pseudomonas oleovorans | |
4-amino-m-cresol | 10% inhibition at 0.05 mM | Pseudomonas oleovorans | |
4-aminoresorcinol | complete inhibition at 0.05 mM | Pseudomonas oleovorans | |
4-Chlorocatechol | complete inhibition at 0.05 mM | Pseudomonas oleovorans | |
4-methylcatechol | complete inhibition at 0.05 mM | Pseudomonas oleovorans | |
Ca2+ | 84% residual activity at 2 mM | Pseudomonas oleovorans | |
Cd2+ | complete inhibition at 2 mM | Pseudomonas oleovorans | |
Co2+ | 47% residual activity at 2 mM | Pseudomonas oleovorans | |
EDTA | 70% residual activity at 2 mM | Pseudomonas oleovorans | |
Fe3+ | 78% residual activity at 2 mM | Pseudomonas oleovorans | |
H2O2 | complete inhibition at 1 mM | Pseudomonas oleovorans | |
K3Fe(CN)6 | 17% residual activity at 1 mM | Pseudomonas oleovorans | |
Mg2+ | 85% residual activity at 2 mM | Pseudomonas oleovorans | |
Mn2+ | 5% residual activity at 2 mM | Pseudomonas oleovorans | |
Mo2+ | 96% residual activity at 2 mM | Pseudomonas oleovorans | |
Ni2+ | 50% residual activity at 2 mM | Pseudomonas oleovorans | |
nitrilotriacetic acid | 86% residual activity at 2 mM | Pseudomonas oleovorans | |
o-phenanthroline | 1% residual activity at 2 mM | Pseudomonas oleovorans | |
protocatechuate | 20% inhibition at 0.05 mM | Pseudomonas oleovorans | |
Zn2+ | complete inhibition at 2 mM | Pseudomonas oleovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0042 | - |
2-Aminophenol | in 50 mM Tris (pH 8.0) at 25°C | Pseudomonas oleovorans | |
0.71 | - |
O2 | in 50 mM Tris (pH 8.0) at 25°C | Pseudomonas oleovorans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | ferrous iron is the sole cofactor, 100% activity at 2 mM | Pseudomonas oleovorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
2 * 39000 + 2 * 35000, SDS-PAGE | Pseudomonas oleovorans |
39000 | - |
2 * 39000 + 2 * 35000, SDS-PAGE | Pseudomonas oleovorans |
140000 | - |
gel filtration | Pseudomonas oleovorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas oleovorans | - |
- |
- |
Pseudomonas oleovorans JS45 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ethanol precipitation, MonoQ column chromatography, and Sephacryl S-300 gel filtration | Pseudomonas oleovorans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.5 | - |
crude extract, in 50 mM Tris (pH 8.0) at 25°C | Pseudomonas oleovorans |
21.6 | - |
purified enzyme, in 50 mM Tris (pH 8.0) at 25°C | Pseudomonas oleovorans |
Storage Stability | Organism |
---|---|
-20°C, purified enzyme in 50 mM MOPS at pH 7.3 in the presence of 10% (v/v) glycerol, several months, no loss of activity | Pseudomonas oleovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-amino-4-chlorophenol + O2 | 15% activity compared to 2-aminophenol | Pseudomonas oleovorans | ? | - |
? | |
2-amino-4-chlorophenol + O2 | 15% activity compared to 2-aminophenol | Pseudomonas oleovorans JS45 | ? | - |
? | |
2-amino-m-cresol + O2 | 1% activity compared to 2-aminophenol | Pseudomonas oleovorans | ? | - |
? | |
2-amino-m-cresol + O2 | 1% activity compared to 2-aminophenol | Pseudomonas oleovorans JS45 | ? | - |
? | |
2-aminophenol + O2 | 100% activity | Pseudomonas oleovorans | 2-aminomuconate 6-semialdehyde | - |
? | |
2-aminophenol + O2 | 100% activity | Pseudomonas oleovorans JS45 | 2-aminomuconate 6-semialdehyde | - |
? | |
6-amino-m-cresol + O2 | 25% activity compared to 2-aminophenol | Pseudomonas oleovorans | ? | - |
? | |
catechol + O2 | 13% activity compared to 2-aminophenol | Pseudomonas oleovorans | ? | - |
? | |
catechol + O2 | 13% activity compared to 2-aminophenol | Pseudomonas oleovorans JS45 | ? | - |
? | |
additional information | 3-methylcatechol, 4-methylcatechol, 3-chlorocatechol, 4-chlorocatechol, 1,2,3-trihydroxybenzene, 1,2,4-trihydroxybenzene, 4-aminoresorcinol, 2-amino-p-cresol, 2,3-dihydroxybenzoic acid, protocatechuate, 2-methoxyphenol, 4-amino-3-hydroxybenzoic acid, 3-amino-4-hydroxybenzoic acid, 3-hydroxyanthranilic acid, and 4-amino-m-cresol are no substrates | Pseudomonas oleovorans | ? | - |
? | |
additional information | 3-methylcatechol, 4-methylcatechol, 3-chlorocatechol, 4-chlorocatechol, 1,2,3-trihydroxybenzene, 1,2,4-trihydroxybenzene, 4-aminoresorcinol, 2-amino-p-cresol, 2,3-dihydroxybenzoic acid, protocatechuate, 2-methoxyphenol, 4-amino-3-hydroxybenzoic acid, 3-amino-4-hydroxybenzoic acid, 3-hydroxyanthranilic acid, and 4-amino-m-cresol are no substrates | Pseudomonas oleovorans JS45 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 2 * 39000 + 2 * 35000, SDS-PAGE | Pseudomonas oleovorans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
the enzyme loses 50% of its activity upon incubation at 60°C for 5 min | Pseudomonas oleovorans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
147 | - |
2-Aminophenol | in 50 mM Tris (pH 8.0) at 25°C | Pseudomonas oleovorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | - |
Pseudomonas oleovorans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10.5 | - |
Pseudomonas oleovorans |