Any feedback?
Literature summary for 1.13.11.B6 extracted from
- Secretion of two novel enzymes, manganese 9S-lipoxygenase and epoxy alcohol synthase, by the rice pathogen Magnaporthe salvinii (2013), J. Lipid Res., 54, 762-775.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression in Pichia pastoris | Nakataea oryzae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| L350M | site-directed mutagenesis, the mutant shows increased specificity for 13S-lipoxygenation, EC 1.13.11.12, altered substrate specificity/regiospecific activity, overview | Nakataea oryzae |
| additional information | replacement Leu350 in 9S-MnLOX and changes the regiospecific oxidation of 18:2 n-6 in a consistent way, but the n-3 double bond of 18:3 n-3 can reduce this effect | Nakataea oryzae |
General Stability
| General Stability | Organism |
|---|---|
| 9S-LOX activity is less robust during expression and purification, possibly due to proteolysis, stability is not improved by addition of 0.001 mM pepstatin A, 0.001 mM leupeptin, or 1 mM EDTA to the growth medium | Nakataea oryzae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0037 | - |
(9Z,12Z)-octadeca-9,12-dienoic acid | pH 9.0, 22°C | Nakataea oryzae |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | the enzyme is secreted | Nakataea oryzae | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | Mn:Fe ratio is 1:0.05 | Nakataea oryzae | |
| Mn2+ | manganese 9S-lipoxygenase, 9S-LOX contains catalytic manganese, Mn:protein ratio is about 0.2:1, while the Mn:Fe ratio is 1:0.05 | Nakataea oryzae | |
| additional information | the catalytic metal of the 9S-LOX is manganese and not iron | Nakataea oryzae |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | - |
x * 80000-90000, recombinant glycosylated enzyme, SDS-PAGE, x * 66000, recombinant deglycosylated enzyme, SDS-PAGE | Nakataea oryzae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Nakataea oryzae | - |
- |
- |
| Nakataea oryzae CBS 288.54 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Pichia pastoris by hydrophobic interaction chromatography, dialfiltration, and gel filtration | Nakataea oryzae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Nakataea oryzae | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| purified recombinant 9S-MnLOX can be stored for several weeks at 4°C with retained activity | Nakataea oryzae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (9E,11Z,15E)-octadeca-9,11,15-trienoic acid + O2 | - |
Nakataea oryzae | ? | - |
? | |
| (9E,11Z,15E)-octadeca-9,11,15-trienoic acid + O2 | - |
Nakataea oryzae CBS 288.54 | ? | - |
? | |
| (9Z,12Z)-octadeca-9,12-dienoic acid + O2 | alpha-linoleate is converted via two intermediates, (9Z,11S,12Z)-11-hydroperoxy-9,12-octadecenoate and (9Z,11E,13R)-13-hydroperoxy-9,12-octadecadienoate, which are isomerized to the end product, probably after oxidation to peroxyl radicals, beta-fragmentation, and oxygen insertion at C-9 | Nakataea oryzae | (9S,10E,12Z)-9-hydroperoxy-octadeca-10,12-dienoate | - |
? | |
| (9Z,12Z)-octadeca-9,12-dienoic acid + O2 | alpha-linoleate is converted via two intermediates, (9Z,11S,12Z)-11-hydroperoxy-9,12-octadecenoate and (9Z,11E,13R)-13-hydroperoxy-9,12-octadecadienoate, which are isomerized to the end product, probably after oxidation to peroxyl radicals, beta-fragmentation, and oxygen insertion at C-9 | Nakataea oryzae CBS 288.54 | (9S,10E,12Z)-9-hydroperoxy-octadeca-10,12-dienoate | - |
? | |
| (9Z,12Z,15Z)-octadeca-9,12,15-trienoic acid + O2 | gamma-linoleate is oxidized at C-9, C-11, and C-13 | Nakataea oryzae | ? | - |
? | |
| (9Z,12Z,15Z)-octadeca-9,12,15-trienoic acid + O2 | gamma-linoleate is oxidized at C-9, C-11, and C-13 | Nakataea oryzae CBS 288.54 | ? | - |
? | |
| additional information | cf. EC 1.13.11.45, LC- and GC-MS analysis product analysis, overview. Authentic (11S)-hydroperoxyoctadecadienoate is rapidly transformed to 9-hydroperoxyoctadecadienoate by 9S-MnLOX, and only traces of 13-hydroperoxyoctadecadienoate. 9S-MnLOX also partly transforms authentic (13R)-hydroperoxyoctadecadienoate to (9S)-hydroperoxyoctadecadienoate | Nakataea oryzae | ? | - |
? | |
| additional information | cf. EC 1.13.11.45, LC- and GC-MS analysis product analysis, overview. Authentic (11S)-hydroperoxyoctadecadienoate is rapidly transformed to 9-hydroperoxyoctadecadienoate by 9S-MnLOX, and only traces of 13-hydroperoxyoctadecadienoate. 9S-MnLOX also partly transforms authentic (13R)-hydroperoxyoctadecadienoate to (9S)-hydroperoxyoctadecadienoate | Nakataea oryzae CBS 288.54 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 80000-90000, recombinant glycosylated enzyme, SDS-PAGE, x * 66000, recombinant deglycosylated enzyme, SDS-PAGE | Nakataea oryzae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| linoleate 9 S-lipoxygenase | - |
Nakataea oryzae |
| manganese 9S-lipoxygenase | - |
Nakataea oryzae |
| S-LOX | - |
Nakataea oryzae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at | Nakataea oryzae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8.3 | - |
(9Z,12Z)-octadeca-9,12-dienoic acid | pH 9.0, 22°C | Nakataea oryzae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
assay at | Nakataea oryzae |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | 9S-LOX contains catalytic manganese, and its sequence can be aligned with 77% identity to 13R-LOX with catalytic manganese lipoxygenase of the Take-all fungus. Alterations in the Sloane determinant of 9S-LOX and 13R-MnLOX with larger and smaller hydrophobic residues interconvert the regiospecifi c oxidation of 18:2 n-6, presumably by altering the substrate position in relation to oxygen insertion | Nakataea oryzae |
| additional information | 9S-MnLOX catalyzes hydrogen abstraction at C-11 and oxygenation at C-9 and C-11 in a suprafacial manner in analogy with 13R-MnLOX, EC 1.13.11.45 | Nakataea oryzae |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.45 | - |
(9Z,12Z)-octadeca-9,12-dienoic acid | pH 9.0, 22°C | Nakataea oryzae | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
