KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
O2 | - |
Cyberlindnera mrakii | |
0.59 | - |
3-Nitro-2-butanol | anionic form of substrate | Cyberlindnera mrakii | |
0.59 | - |
3-Nitro-2-butanol | anionic form of the substrate | Cyberlindnera mrakii | |
1.04 | - |
3-Nitro-2-pentanol | anionic form of substrate | Cyberlindnera mrakii | |
1.04 | - |
3-Nitro-2-pentanol | anionic form of the substrate | Cyberlindnera mrakii | |
1.61 | - |
2-Nitropropane | anionic form of substrate | Cyberlindnera mrakii | |
1.61 | - |
2-Nitropropane | anionic form of the substrate | Cyberlindnera mrakii | |
3.13 | - |
nitroethane | anionic form of substrate | Cyberlindnera mrakii | |
3.13 | - |
nitroethane | anionic form of the substrate | Cyberlindnera mrakii | |
3.23 | - |
1-Nitropropane | anionic form of substrate | Cyberlindnera mrakii | |
3.23 | - |
1-Nitropropane | anionic form of the substrate | Cyberlindnera mrakii | |
4.2 | - |
3-Nitro-2-butanol | - |
Cyberlindnera mrakii | |
4.2 | - |
3-Nitro-2-butanol | neutral form of the substrate | Cyberlindnera mrakii | |
6.8 | - |
nitroethane | neutral form of substrate | Cyberlindnera mrakii | |
6.8 | - |
nitroethane | neutral form of the substrate | Cyberlindnera mrakii | |
21.3 | - |
2-Nitropropane | neutral form of substrate | Cyberlindnera mrakii | |
21.3 | - |
2-Nitropropane | neutral form of the substrate | Cyberlindnera mrakii | |
24.3 | - |
nitroethane | neutral form of substrate | Cyberlindnera mrakii | |
24.3 | - |
nitroethane | neutral form of the substrate | Cyberlindnera mrakii | |
25.6 | - |
1-Nitropropane | neutral form of substrate | Cyberlindnera mrakii | |
25.6 | - |
1-Nitropropane | neutral form of the substrate | Cyberlindnera mrakii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | enzyme contains 1.09 gatom of iron per mol of enzyme | Cyberlindnera mrakii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
sedimentation equilibrium centrifugation | Cyberlindnera mrakii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Cyberlindnera mrakii | enzyme catalyzes the oxygenative denitrification of anionic nitroalkanes much more effectively than that of the neutral ones | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cyberlindnera mrakii | - |
- |
- |
Cyberlindnera mrakii | Q12723 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Cyberlindnera mrakii |
to homogeneity | Cyberlindnera mrakii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
397 | - |
- |
Cyberlindnera mrakii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-nitropropane + O2 | 57.9% of activity with 2-nitropropane | Cyberlindnera mrakii | propionaldehyde + HNO2 | - |
? | |
1-nitropropane + O2 | 57.9% of activity with 2-nitropropane (anionic form) | Cyberlindnera mrakii | propionaldehyde + HNO2 | - |
? | |
2-nitro-1-butanol + O2 | 31.5% of the activity with 2-nitropropane | Cyberlindnera mrakii | 1-hydroxy-butane-2-one + HNO2 | - |
? | |
2-nitro-1-butanol + O2 | 2.7% of the activity with 2-nitropropane | Cyberlindnera mrakii | 1-hydroxy-butane-2-one + HNO2 | - |
? | |
2-nitro-1-butanol + O2 | 2.7% of the activity with 2-nitropropane (anionic form) | Cyberlindnera mrakii | 1-hydroxy-butane-2-one + HNO2 | - |
? | |
2-nitro-1-butanol + O2 | 31.5% of the activity with 2-nitropropane (anionic form) | Cyberlindnera mrakii | 1-hydroxy-butane-2-one + HNO2 | - |
? | |
2-nitro-1-propanol + O2 | 7.7% of activity with 2-nitropropane | Cyberlindnera mrakii | 1-hydroxy-propane-2-one + HNO2 | - |
? | |
2-nitro-1-propanol + O2 | 7.7% of activity with 2-nitropropane (anionic form) | Cyberlindnera mrakii | 1-hydroxy-propane-2-one + HNO2 | - |
? | |
2-nitropropane + O2 | - |
Cyberlindnera mrakii | acetone + HNO2 | - |
? | |
3-nitro-2-butanol + O2 | 26.7% of the activity with 2-nitropropane | Cyberlindnera mrakii | 3-hydroxy-butane-2-one + HNO2 | - |
? | |
3-nitro-2-butanol + O2 | 26.7% of the activity with 2-nitropropane (anionic form) | Cyberlindnera mrakii | 3-hydroxy-butane-2-one + HNO2 | - |
? | |
3-nitro-2-pentanol + O2 | 32.3% of the activity with 2-nitropropane | Cyberlindnera mrakii | 2-hydroxy-pentane-3-one + HNO2 | - |
? | |
3-nitro-2-pentanol + O2 | 32.3% of the activity with 2-nitropropane (anionic form) | Cyberlindnera mrakii | 2-hydroxy-pentane-3-one + HNO2 | - |
? | |
3-nitropropionic acid + O2 | 25.5% of the activity with 2-nitropropane | Cyberlindnera mrakii | ? | - |
? | |
3-nitropropionic acid + O2 | 25.5% of the activity with 2-nitropropane (anionic form) | Cyberlindnera mrakii | ? | - |
? | |
additional information | enzyme catalyzes the oxygenative denitrification of anionic nitroalkanes much more effectively than that of the neutral ones | Cyberlindnera mrakii | ? | - |
? | |
nitrocyclohexane + O2 | 1.5% of the activity with 2-nitropropane | Cyberlindnera mrakii | cyclohexanone + HNO2 | - |
? | |
nitrocyclohexane + O2 | 1.5% of the activity with 2-nitropropane (anionic form) | Cyberlindnera mrakii | cyclohexanone + HNO2 | - |
? | |
nitroethane + O2 | 4.2% of the activity with 2-nitropropane | Cyberlindnera mrakii | acetaldehyde + HNO2 | - |
? | |
nitroethane + O2 | 4.2% of the activity with 2-nitropropane (anionic form) | Cyberlindnera mrakii | acetaldehyde + HNO2 | - |
? | |
nitromethane + O2 | 4.2% of the activity with 2-nitropropane activity | Cyberlindnera mrakii | formaldehyde + HNO2 | - |
? | |
nitromethane + O2 | 4.2% of the activity with 2-nitropropane activity (anionic form) | Cyberlindnera mrakii | formaldehyde + HNO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 42000, SDS-PAGE | Cyberlindnera mrakii |
Synonyms | Comment | Organism |
---|---|---|
2-nitropropane dioxygenase | - |
Cyberlindnera mrakii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
oxidation of anionic form of 2-nitropropane | Cyberlindnera mrakii |
8 | - |
oxidation of neutral form of 2-nitropropane | Cyberlindnera mrakii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | contains 1 mol FAD per mol of enzynme | Cyberlindnera mrakii | |
FAD | contains 1 mol FAD per mol of enzyme | Cyberlindnera mrakii |