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Literature summary for 1.13.12.16 extracted from

  • Kido, T.; Soda, K.; Suzuki, T.; Asada, K.
    A new oxygenase, 2-nitropropane dioxygenase of Hansenula mrakii. Enzymologic and spectrophotometric properties (1976), J. Biol. Chem., 251, 6994-7000.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2,2'-dipyridyl
-
Cyberlindnera mrakii
2,4,6-tripyridyl-s-triazine
-
Cyberlindnera mrakii
o-phenanthroline
-
Cyberlindnera mrakii

General Stability

General Stability Organism
denaturation by sodium dithionite Cyberlindnera mrakii
gradual and irreversible loss of activity after treatment with 1% sodium lauryl sulfate or 6 M guanidine HCl, activity is reduced to 50% of its initial activity after 50 min at 37°C, complete loss of activity after 10 h Cyberlindnera mrakii
immediate denaturation with 8 M urea or thiourea Cyberlindnera mrakii

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
Cyberlindnera mrakii
8-hydroxyquinoline strong inhibition Cyberlindnera mrakii
cysteine marked decrease in enzyme activity Cyberlindnera mrakii
GSH marked decrease in enzyme activity Cyberlindnera mrakii
HgCl2 moderate and potent inhibitor Cyberlindnera mrakii
additional information EDTA does not inhibit the enzyme significantly. Iodoacetate is almost ineffective Cyberlindnera mrakii
N-ethylmaleimide
-
Cyberlindnera mrakii
NaHSO3
-
Cyberlindnera mrakii
NEM
-
Cyberlindnera mrakii
Nitromethane inhibits noncompetitively Cyberlindnera mrakii
p-chloromercuribenzoate
-
Cyberlindnera mrakii
PCMB
-
Cyberlindnera mrakii
Tiron i.e. pyrocatechol-3,5-disulfonate disodium salt; i.e. pyrocatechol-3,5-disulfonate disodium salt, strong inhibition Cyberlindnera mrakii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
O2
-
Cyberlindnera mrakii
4.2
-
3-Nitro-2-butanol neutral form of substrate Cyberlindnera mrakii
6.8
-
nitroethane
-
Cyberlindnera mrakii
6.8
-
3-Nitro-2-pentanol
-
Cyberlindnera mrakii
21.3
-
2-Nitropropane
-
Cyberlindnera mrakii
24.3
-
nitroethane
-
Cyberlindnera mrakii
25.6
-
1-Nitropropane
-
Cyberlindnera mrakii

Metals/Ions

Metals/Ions Comment Organism Structure
Fe3+ contains 1 g atom of non-heme iron per mol of enzyme Cyberlindnera mrakii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
1 * 25000 + 1 * 39000, SDS-PAGE Cyberlindnera mrakii
39000
-
1 * 25000 + 1 * 39000, SDS-PAGE Cyberlindnera mrakii
60000
-
gel filtration Cyberlindnera mrakii
64000
-
equilibrium sedimentation Cyberlindnera mrakii

Organism

Organism UniProt Comment Textmining
Cyberlindnera mrakii
-
-
-
Cyberlindnera mrakii
-
IF0 0895
-
Cyberlindnera mrakii IF0 0895
-
IF0 0895
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity Cyberlindnera mrakii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-nitropropane + O2 23.4% of the activity with 2-nitropropane Cyberlindnera mrakii propionaldehyde + HNO2
-
?
1-nitropropane + O2 23.4% of the activity with 2-nitropropane Cyberlindnera mrakii IF0 0895 propionaldehyde + HNO2
-
?
2-nitropropane + O2
-
Cyberlindnera mrakii acetone + HNO2
-
?
2-nitropropane + O2
-
Cyberlindnera mrakii IF0 0895 acetone + HNO2
-
?
3-nitro-2-butanol + O2 13% of the activity with 2-nitropropane Cyberlindnera mrakii 3-hydroxy-butane-2-one + HNO2
-
?
3-nitro-2-butanol + O2 slight oxidation Cyberlindnera mrakii 3-hydroxy-butane-2-one + HNO2
-
?
3-nitro-2-pentanol + O2 40.6% of the activity with 2-nitropropane Cyberlindnera mrakii 2-hydroxy-pentane-3-one + HNO2
-
?
3-nitropropionic acid + O2 11.7% of the activity with 2-nitropropane Cyberlindnera mrakii ?
-
?
additional information sodium dithionite also reduces both the enzyme-bound FAD and Fe3+ under anaerobic conditions Cyberlindnera mrakii ?
-
?
additional information sodium dithionite also reduces both the enzyme-bound FAD and Fe3+ under anaerobic conditions Cyberlindnera mrakii IF0 0895 ?
-
?
nitroethane + O2 88% of the activity with nitroethane Cyberlindnera mrakii acetaldehyde + HNO2
-
?
nitroethane + O2 88% of the activity with nitroethane Cyberlindnera mrakii IF0 0895 acetaldehyde + HNO2
-
?
nitromethane + O2 no activity Cyberlindnera mrakii formaldehyde + HNO2
-
?
nitromethane + O2 is not a substrate, under anaerobic conditions. The aerobic dialysis of the enzyme treated with nitromethane causes reoxidation of only the Fe2+ Cyberlindnera mrakii formaldehyde + HNO2
-
?

Subunits

Subunits Comment Organism
dimer 1 * 25000 + 1 * 39000, SDS-PAGE Cyberlindnera mrakii

Synonyms

Synonyms Comment Organism
2-nitropropane dioxygenase
-
Cyberlindnera mrakii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Cyberlindnera mrakii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 45
-
Cyberlindnera mrakii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
when the enzyme is dialyzed against 10 mM potassium phosphate buffer (pH 7.0) immediately after reduction by dithionite, the absorption spectrum similar to that of the native enzyme appears with concomitant restoration of approximately 80% of the activity Cyberlindnera mrakii
8
-
-
Cyberlindnera mrakii

pH Range

pH Minimum pH Maximum Comment Organism
7 8.5
-
Cyberlindnera mrakii
7 8.5 when the enzyme is acidified to pH 3.0 and treated in the same way, the prosthetic groups do not dissociate from the protein and almost full activity remained Cyberlindnera mrakii

Cofactor

Cofactor Comment Organism Structure
FAD contains 0.95 mol of FAD per mol of enzyme Cyberlindnera mrakii
FAD contains 0.95 mol of FAD per mol of enzyme. The enzyme-bound FAD is reduced by 2-nitropropane under anaerobic conditions Cyberlindnera mrakii