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Literature summary for 1.13.12.16 extracted from

  • Kido, T.; Yamamoto, T.; Soda, K.
    Purification and properties of nitroalkane-oxidizing enzyme from Hansenula mrakii (1976), J. Bacteriol., 126, 1261-1265.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.12.16

Organism

Organism UniProt Comment Textmining
Cyberlindnera mrakii
-
-
-
Cyberlindnera mrakii
-
 IFO 0895
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cyberlindnera mrakii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.5
-
-
 Cyberlindnera mrakii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-nitropropane + O2
-
 Cyberlindnera mrakii propionaldehyde + HNO2
-
 ?
2-nitropropane + O2
-
 Cyberlindnera mrakii acetone + HNO2
-
 ?
additional information no activity, nitromethane is inert to the enzyme. The nitroalkanes are not oxidized under anaerobic conditions Cyberlindnera mrakii ?
-
 ?
nitroethane + O2
-
 Cyberlindnera mrakii acetaldehyde + HNO2
-
 ?
nitromethane + O2 no activity Cyberlindnera mrakii formaldehyde + HNO2
-
 ?

Subunits

Subunits Comment Organism
More purified enzyme shows a single protein band upon disc-gel electrophoresis Cyberlindnera mrakii

Synonyms

Synonyms Comment Organism
nitroalkane-oxidizing enzyme
-
 Cyberlindnera mrakii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Cyberlindnera mrakii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at, pH 8.0 for 2-nitropropane and 1-nitropropane and pH 7.0 for nitroethane Cyberlindnera mrakii
8
-
 assay at, pH 8.0 for 2-nitropropane and 1-nitropropane and pH 7.0 for nitroethane Cyberlindnera mrakii