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Literature summary for 1.13.12.18 extracted from

  • Dunlap, J.C.; Hastings, J.W.
    The biological clock in Gonyaulax controls luciferase activity by regulating turnover (1981), J. Biol. Chem., 256, 10509-10518.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0025
-
dinoflagellate luciferin in 0.2 M sodium phosphate, 0.25 mM EDTA, 0.1 mg/ml of bovine serum albumin at pH 6.3, temperature not specified in the publication Lingulodinium polyedra

Metals/Ions

Metals/Ions Comment Organism Structure
additional information luciferase is not a Mn2+, Mg2+ , or Ca2+ metalloprotein Lingulodinium polyedra

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
130000
-
3 * 130000, SDS-PAGE Lingulodinium polyedra
420000
-
gel filtration Lingulodinium polyedra

Organism

Organism UniProt Comment Textmining
Lingulodinium polyedra
-
formerly Gonyaulax polyedra
-
Lingulodinium polyedra 70
-
formerly Gonyaulax polyedra
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation Lingulodinium polyedra

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dinoflagellate luciferin + O2
-
Lingulodinium polyedra oxidized dinoflagellate luciferin + H2O + hv
-
?
dinoflagellate luciferin + O2
-
Lingulodinium polyedra 70 oxidized dinoflagellate luciferin + H2O + hv
-
?

Subunits

Subunits Comment Organism
homotrimer 3 * 130000, SDS-PAGE Lingulodinium polyedra

Expression

Organism Comment Expression
Lingulodinium polyedra luciferase activity in cell-free extracts of Gonyaulax polyedm undergoes a cyclic daily change such that activities of extracts made in the middle of the night phase may be 10 times greater than in extracts of day phase cells. The circadian rhythm of luciferase activity is a result of biological clock-controlled synthesis and/or degradation of the luciferase polypeptide up