KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
dinoflagellate luciferin | in 0.2 M sodium phosphate, 0.25 mM EDTA, 0.1 mg/ml of bovine serum albumin at pH 6.3, temperature not specified in the publication | Lingulodinium polyedra |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | luciferase is not a Mn2+, Mg2+ , or Ca2+ metalloprotein | Lingulodinium polyedra |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
130000 | - |
3 * 130000, SDS-PAGE | Lingulodinium polyedra |
420000 | - |
gel filtration | Lingulodinium polyedra |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lingulodinium polyedra | - |
formerly Gonyaulax polyedra | - |
Lingulodinium polyedra 70 | - |
formerly Gonyaulax polyedra | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation | Lingulodinium polyedra |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dinoflagellate luciferin + O2 | - |
Lingulodinium polyedra | oxidized dinoflagellate luciferin + H2O + hv | - |
? | |
dinoflagellate luciferin + O2 | - |
Lingulodinium polyedra 70 | oxidized dinoflagellate luciferin + H2O + hv | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotrimer | 3 * 130000, SDS-PAGE | Lingulodinium polyedra |
Organism | Comment | Expression |
---|---|---|
Lingulodinium polyedra | luciferase activity in cell-free extracts of Gonyaulax polyedm undergoes a cyclic daily change such that activities of extracts made in the middle of the night phase may be 10 times greater than in extracts of day phase cells. The circadian rhythm of luciferase activity is a result of biological clock-controlled synthesis and/or degradation of the luciferase polypeptide | up |